18658-1-AP

antibody from Proteintech Group

Targeting: NR5A1 AD4BP, ELP, FTZ1, FTZF1, hSF-1, SF-1, SF1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

18658-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#18658-1-AP, RRID:AB_10596916

Product name

NR5A1 antibody

Antibody type

Polyclonal

Description

NR5A1 antibody (Cat. #18658-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Targeting the NR5A1-KISS1R-EMT axis with a small-molecule inhibitor suppresses lung adenocarcinoma progression and metastasis.

Wang D, Hui K, Yuan C, Lan W, Liu L, Yu Z, Li X, Shen X, Liu B, Jiang X
Cellular signalling 2026 Jul;143:112453

---

Honghua Xiaoyao tablets ameliorate premenstrual syndrome by modulating HPA axis and restoring ovarian estrogen synthesis to attenuate hippocampal neuroinflammation.

Chen K, Gao S, Cai B, Ma Z, Liu X, Chen W, Lin N, Zhang Y
Journal of ethnopharmacology 2026 Jan 30;355(Pt B):120688

---

Genome-wide identification of FTZ-F1 genes in Chlamys farreri and analysis of ChIP-seq-based binding sites and potential target genes.

Fan Q, Liu P, Miao J, Han L, Lei K, Fu R, Li X, Pan L
Comparative biochemistry and physiology. Part D, Genomics & proteomics 2025 Sep;55:101458

---

Trps1 targets Scarb1 to regulate cholesterol acquisition in mouse Leydig cells.

Lian X, Lin Z, Hu W, Chen Z, Luo S, Liao S, Liu Y, Wang S, Sun J
Reproductive biology 2025 Sep;25(3):101046

---

NEK2 Contributes to the Protection Against Cryptorchidism Outcomes.

Zhou X, Ye S, Wang X, Liang Z, Qian N, Ji L, Xian H, Wang Z, Ge W
Birth defects research 2025 May;117(5):e2485

---

NR5A1 Deficiency Leads to Decidualization Dysregulation of Stromal Cells and Recurrent Spontaneous Abortion During Early Pregnancy.

Dai J, Jiang H, Liu D, Dong Z, Hu L, He F, Yu T
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2025 Jun 30;39(12):e70752

---

DEHP-mediated oxidative stress leads to impaired testosterone synthesis in Leydig cells through the cAMP/PKA/SF-1/StAR pathway.

Yang L, Liu S, Song P, Liu Z, Peng Z, Kong D, Zhou J, Yan X, Ma K, Yu Y, Liu X, Dong Q
Environmental pollution (Barking, Essex : 1987) 2025 Feb 1;366:125503

---

Gut microbiota-metabolite crosstalk mediates icariin's protection against obesity-induced spermatogenic dysfunction through NF-κB/AMH axis in Sertoli cells.

Huang Q, Ji L, Meng Z, Huang K, Lei S, Xue H, Zhou Q, Tian Y, Chen T, Lei X, Huang H
Phytomedicine : international journal of phytotherapy and phytopharmacology 2025 Dec;149:157490

---

Stress-Induced Disruption of Circadian and Neuroimmune Networks in Lacrimal Glands Drives Dry Eye Pathogenesis.

Ba M, Huang D, Yang T, Xuan S, Zhang W, Qi D, Pei X, Lu D, Huang S, Yang J, Liu X, Li Z
Investigative ophthalmology & visual science 2025 Dec 1;66(15):53

---

Chronic Restraint Stress Misaligns Corneal Clock via β-Adrenergic and Glucocorticoid Signaling, Altering Epithelial, Neural, Immune, Metabolic States.

Xuan S, Yang T, Ba M, Zhang W, Yang J, Pei X, Qi D, Lu D, Huang S, Li Z
Investigative ophthalmology & visual science 2025 Dec 1;66(15):50

---

Pharmacogenomic analysis in adrenocortical carcinoma reveals genetic features associated with mitotane sensitivity and potential therapeutics.

Zhang J, Wu L, Su T, Liu H, Jiang L, Jiang Y, Wu Z, Chen L, Li H, Zheng J, Sun Y, Peng H, Han R, Ning G, Ye L, Wang W
Frontiers in endocrinology 2024;15:1365321

---

GRP78/IRE1 and cGAS/STING pathway crosstalk through CHOP facilitates iodoacetic acid-mediated testosterone decline.

Mou L, Sun D, Qu J, Tan X, Wang S, Zeng Q, Liu C
Journal of hazardous materials 2024 Sep 5;476:135101

---

The senolytic drug ABT-263 accelerates ovarian aging in older female mice.

Xia X, Yang Y, Liu P, Chen L, Dai X, Xue P, Wang Y
Scientific reports 2024 Oct 5;14(1):23178

---

LncRNA NORFA promotes the synthesis of estradiol and inhibits the apoptosis of sow ovarian granulosa cells through SF-1/CYP11A1 axis.

Guo Z, Zeng Q, Li Q, Shan B, Huo Y, Shi X, Li Q, Du X
Biology direct 2024 Nov 10;19(1):107

---

Luoshi Neiyi Prescription inhibits estradiol synthesis and inflammation in endometriosis through the HIF1A/EZH2/SF-1 pathway.

Wu L, Lan D, Sun B, Su R, Pei F, Kuang Z, Su Y, Lin S, Wang X, Zhang S, Chen X, Jia J, Zeng C
Journal of ethnopharmacology 2024 Dec 5;335:118659

---

Seasonal changes of vitamin D(3) and ovarian steroidogenesis in the wild ground squirrels (Citellus dauricus Brandt).

Lu W, Zhou Y, Liu Y, Zhang H, Yuan Z, Han Y, Weng Q
The Journal of steroid biochemistry and molecular biology 2023 Nov;234:106385

---

Expression of cholesterol synthesis and steroidogenic markers in females of the Chinese brown frog (Rana dybowskii) during prespawning and prehibernation.

Chen Y, Liu Y, Wang Y, Zhang Y, Xie W, Zhang H, Weng Q, Xu M
American journal of physiology. Regulatory, integrative and comparative physiology 2023 Dec 1;325(6):R750-R758

---

Identification of Key Differentially Methylated/Expressed Genes and Pathways for Ovarian Endometriosis by Bioinformatics Analysis.

Zhang H, Wu J, Li Y, Jin G, Tian Y, Kang S
Reproductive sciences (Thousand Oaks, Calif.) 2022 May;29(5):1630-1643

---

Nicotinamide N-Methyltransferase inhibits HBV replication by suppressing NR5A1 expression invitro.

Fan SY, Long SY, Liu JJ, Zhang WL, Hu JL
Biochemical and biophysical research communications 2022 Jul 23;614:70-77

---

Icariin promotes mouse Leydig cell testosterone synthesis via the Esr1/Src/Akt/Creb/Sf-1 pathway.

Sun J, Xu W, Zheng S, Lv C, Lin J, Chen S, Qiu Y, Jiang X, Draz E, Wang S
Toxicology and applied pharmacology 2022 Apr 15;441:115969

---

Pde8b haploinsufficiency in mice is associated with modest adrenal defects, impaired steroidogenesis, and male infertility, unaltered by concurrent PKA or Wnt activation.

Leal LF, Szarek E, Berthon A, Nesterova M, Faucz FR, London E, Mercier C, Abu-Asab M, Starost MF, Dye L, Bilinska B, Kotula-Balak M, Antonini SR, Stratakis CA
Molecular and cellular endocrinology 2021 Feb 15;522:111117

---

Screening and Identification of Differential Ovarian Proteins before and after Induced Ovulation via Seminal Plasma in Bactrian Camels.

Wang Q, Zhang Q, Li Y, Zhao X, Zhang Y
Animals : an open access journal from MDPI 2021 Dec 9;11(12)

---

PRMT5 regulates ovarian follicle development by facilitating Wt1 translation.

Chen M, Dong F, Chen M, Shen Z, Wu H, Cen C, Cui X, Bao S, Gao F
eLife 2021 Aug 27;10

---

Seasonal expressions of SF-1, StAR and P450scc in the scent glands of the muskrats (Ondatra zibethicus).

Xie W, Tang Z, Xu L, Zhong J, Zhang H, Han Y, Yuan Z, Weng Q
The Journal of steroid biochemistry and molecular biology 2020 Nov;204:105766

---

Inactivation of Wt1 causes pre-granulosa cell to steroidogenic cell transformation and defect of ovary development†.

Cen C, Chen M, Zhou J, Zhang L, Duo S, Jiang L, Hou X, Gao F
Biology of reproduction 2020 Jun 23;103(1):60-69

---

Transactivation of miR-202-5p by Steroidogenic Factor 1 (SF1) Induces Apoptosis in Goat Granulosa Cells by Targeting TGFβR2.

Ding Q, Jin M, Wang Y, Liu J, Kalds P, Wang Y, Yang Y, Wang X, Chen Y
Cells 2020 Feb 14;9(2)

---

Icariin protects mouse Leydig cell testosterone synthesis from the adverse effects of di(2-ethylhexyl) phthalate.

Sun J, Wang D, Lin J, Liu Y, Xu L, Lv R, Mo K, Lian X, Xie M, Xu S, Wang S
Toxicology and applied pharmacology 2019 Sep 1;378:114612

---

Induction of Sertoli-like cells from human fibroblasts by NR5A1 and GATA4.

Liang J, Wang N, He J, Du J, Guo Y, Li L, Wu W, Yao C, Li Z, Kee K
eLife 2019 Nov 11;8

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

mouse ovary tissue were subjected to SDS PAGE followed by western blot with 18658-1-AP(NR5A1 antibody) at dilution of 1:1000

Sample type

tissue

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The NR5A1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human NR5A1. This antibody recognizes human,mouse,rat antigen. The NR5A1 antibody has been validated for the following applications: ELISA, WB, IHC analysis.

Appendix

18658-1-AP.pdf

Show more

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human liver using 18658-1-AP(NR5A1 antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue