V5307

antibody from NSJ Bioreagents

Targeting: NR5A1 AD4BP, ELP, FTZ1, FTZF1, hSF-1, SF-1, SF1

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V5307

Provider

NSJ Bioreagents

Product name

SF-1 Antibody / Steroidogenic Factor 1 / NR5A1

Antibody type

Monoclonal

Description

This highly specific SF-1 antibody is suitable for use in Immunohistochemistry applications with human samples.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antibody clone number

SF1/8150R

Vial size

20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)

Concentration

0.2 mg/ml, 1 mg/ml

Storage

Aliquot the SF-1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human testis tissue with SF-1 antibody (clone SF1/8150R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human testis tissue with SF-1 antibody (clone SF1/8150R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE analysis of purified, BSA-free SF-1 antibody (clone SF1/8150R) as confirmation of integrity and purity.