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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- PA5-17529 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TGFBR3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody. This antibody is not cross-reactive with other family members at physiological conditions.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 120.8 µg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TGF-beta Receptor III in extracts from C2C12 cells upon differentiation into myoblasts using TGF-beta Receptor III polyclonal antibody (Product # PA5-17529).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts of Hep G2 (Lane 1), undifferentiated C2C12 (Lane 2), differentiated C2C12 (Lane 3), THP-1 (Lane 4), SH-SY5Y (Lane 5), Mouse Adipose (Lane 6) and Mouse Brain (Lane 7). The blot was probed with TGFBR3 Polyclonal Antibody (Product # PA5-17529, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 100kDa band corresponding to TGFBR3 was observed in HepG2, THP-1, Mouse Adipose and was not observed in SHSY5Y, Mouse Brain. C2C12 showed an upregulation in the expression of TGFBR3 upon differentiation.
