14690-1-AP

antibody from Invitrogen Antibodies

Targeting: AGAP2 CENTG1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14690-1-AP

Provider

Invitrogen Antibodies

Product name

AGAP2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MVKKQRRKK LTTPSKTEGS AGQAEEENFE FLIVSSTGQT WHFEAASFEE RDAWVQAIES QILASLQCCE SSKVKLRTDS QSEAVAIQAI RNAKGNSICV DCGAPNPTWA SLNLGALICI ECSGIHRNLG THLSRVRSLD LDDWPRELTL VLTAIGNDTA NRVWESDTRG RAKPSRDSSR EERESWIRAK YEQLLFLAPL STSEEPLGRQ LWAAVQAQDV ATVLLLLAHA RHGPLDTSVE DPQLRSPLHL AAELAHVVIT QLLLWYGADV AARDAQGRTA LFYARQAGSQ LCADILLQHG CPGEGGSAAT TPSAATTPSI TATPSPRRRS SAASVGRADA PVALV (848-1192 aa encoded by BC028020)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.17 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Rat brain tissue were subjected to SDS PAGE followed by western blot with 14690-1-AP (AGAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Rat brain tissue were subjected to SDS PAGE followed by western blot with 14690-1-AP (AGAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 14690-1-AP (AGAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using AGAP2 antibody 14690-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 14690-1-AP (AGAP2 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 14690-1-AP ( AGAP2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-AGAP2 (IP:14690-1-AP, 4ug; Detection:14690-1-AP 1:500) with mouse brain tissue lysate 4000ug.