Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [7]
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Validation data
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- Product number
- NBP1-89559 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-89559, RRID:AB_11025037
- Product name
- Rabbit Polyclonal ME2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human ME2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Chronic reduction of the cytosolic or mitochondrial NAD(P)-malic enzyme does not affect insulin secretion in a rat insulinoma cell line.
Succinate is the controller of O2-/H2O2 release at mitochondrial complex I : negative modulation by malate, positive by cyanide.
Mitochondrial malic enzyme (ME2) in pancreatic islets of the human, rat and mouse and clonal insulinoma cells.
Mitochondrial malic enzyme (ME2) in pancreatic islets of the human, rat and mouse and clonal insulinoma cells.
Mycobacterium avium-Mycobacterium intracellular complex-induced suppression of T-cell proliferation in vitro by regulation of monocyte accessory cell activity.
Brown LJ, Longacre MJ, Hasan NM, Kendrick MA, Stoker SW, Macdonald MJ
The Journal of biological chemistry 2009 Dec 18;284(51):35359-67
The Journal of biological chemistry 2009 Dec 18;284(51):35359-67
Succinate is the controller of O2-/H2O2 release at mitochondrial complex I : negative modulation by malate, positive by cyanide.
Zoccarato F, Cavallini L, Alexandre A
Journal of bioenergetics and biomembranes 2009 Aug;41(4):387-93
Journal of bioenergetics and biomembranes 2009 Aug;41(4):387-93
Mitochondrial malic enzyme (ME2) in pancreatic islets of the human, rat and mouse and clonal insulinoma cells.
MacDonald MJ, Longacre MJ, Kendrick MA
Archives of biochemistry and biophysics 2009 Aug 15;488(2):100-4
Archives of biochemistry and biophysics 2009 Aug 15;488(2):100-4
Mitochondrial malic enzyme (ME2) in pancreatic islets of the human, rat and mouse and clonal insulinoma cells.
MacDonald MJ, Longacre MJ, Kendrick MA
Archives of biochemistry and biophysics 2009 Aug 15;488(2):100-4
Archives of biochemistry and biophysics 2009 Aug 15;488(2):100-4
Mycobacterium avium-Mycobacterium intracellular complex-induced suppression of T-cell proliferation in vitro by regulation of monocyte accessory cell activity.
Tsuyuguchi I, Kawasumi H, Takashima T, Tsuyuguchi T, Kishimoto S
Infection and immunity 1990 May;58(5):1369-78
Infection and immunity 1990 May;58(5):1369-78
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ME2 Antibody [NBP1-89559] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ME2 Antibody [NBP1-89559] - Analysis in human cell line Daudi.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ME2 Antibody [NBP1-89559] - Analysis using Anti-ME2 antibody NBP1-89559 (A) shows similar pattern to independent antibody NBP1-82434 (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ME2 Antibody [NBP1-89559] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ME2 Antibody [NBP1-89559] - Staining of human duodenum shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ME2 Antibody [NBP1-89559] - Staining of human liver shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ME2 Antibody [NBP1-89559] - Staining in human duodenum and liver tissues using anti-ME2 antibody. Corresponding ME2 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ME2 Antibody [NBP1-89559] - Staining of human lymph node.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ME2 Antibody [NBP1-89559] - Staining of human cerebral cortex.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ME2 Antibody [NBP1-89559] - Staining of human colon.