HPA008880

antibody from Atlas Antibodies

Targeting: ME2

Western blot (Enhanced validation)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA008880

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA008880, RRID:AB_1853702

Product name

Anti-ME2

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

KVISKPISEHKILFLGAGEAALGIANLIVMSMVEN
GLSEQEAQKKIWMFDKYGLLVKGRKAKIDSYQEPF
THSAPESIPDTFEDAVNILKPSTIIGVAGAGRLFT
PDVIRAMASINERPVIFALSNPTA


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References

Submitted references

Chronic reduction of the cytosolic or mitochondrial NAD(P)-malic enzyme does not affect insulin secretion in a rat insulinoma cell line.

Brown LJ, Longacre MJ, Hasan NM, Kendrick MA, Stoker SW, Macdonald MJ
The Journal of biological chemistry 2009 Dec 18;284(51):35359-67

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Succinate is the controller of O 2 − /H2O2 release at mitochondrial complex I : negative modulation by malate, positive by cyanide

Zoccarato F, Cavallini L, Alexandre A
Journal of Bioenergetics and Biomembranes 2009 August;41(4):387-393

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Mitochondrial malic enzyme (ME2) in pancreatic islets of the human, rat and mouse and clonal insulinoma cells.

MacDonald MJ, Longacre MJ, Kendrick MA
Archives of biochemistry and biophysics 2009 Aug 15;488(2):100-4

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Mitochondrial malic enzyme (ME2) in pancreatic islets of the human, rat and mouse and clonal insulinoma cells.

MacDonald MJ, Longacre MJ, Kendrick MA
Archives of biochemistry and biophysics 2009 Aug 15;488(2):100-4

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Independent antibody validation

Main image

Experimental details

Western blot analysis using Anti-ME2 antibody HPA008880 (A) shows similar pattern to independent antibody HPA008247 (B).

Antibody #2 product nr

HPA008247

Antibody provider

Atlas Antibodies

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Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human duodenum and liver tissues using Anti-ME2 antibody. Corresponding ME2 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human duodenum shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver shows low expression as expected.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human lymph node using Anti-ME2 antibody HPA008880.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colon using Anti-ME2 antibody HPA008880.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex using Anti-ME2 antibody HPA008880.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis using Anti-ME2 antibody HPA008880.

Sample type

HUMAN