Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [3]
- Immunohistochemistry [8]
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Validation data
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- Product number
- LS-C746926 - Provider product page
- Provider
- LSBio
- Product name
- SIRT1 / Sirtuin 1 Antibody LS-C746926
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using SIRT1 antibody at 1:500 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 10s.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis of extracts from normal (control) and SIRT1 knockout (KO) HeLa cells, using SIRT1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 10s.
Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug SIRT1 antibody. Western blot was performed from the immunoprecipitate using SIRT1 antibody at a dilition of 1:1000.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug SIRT1 antibody. Western blot was performed from the immunoprecipitate using SIRT1 antibody at a dilition of 1:1000.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug SIRT1 antibody. Western blot was performed from the immunoprecipitate using SIRT1 antibody at a dilition of 1:1000.
Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse testis using SIRT1 antibody at dilution of 1:100 (40x lens).
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded rat liver using SIRT1 antibody at dilution of 1:100 (40x lens).
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded rat testis using SIRT1 antibody at dilution of 1:100 (40x lens).
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon carcinoma using SIRT1 antibody at dilution of 1:100 (40x lens).
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse testis using SIRT1 antibody at dilution of 1:100 (40x lens).
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded rat liver using SIRT1 antibody at dilution of 1:100 (40x lens).
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded rat testis using SIRT1 antibody at dilution of 1:100 (40x lens).
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon carcinoma using SIRT1 antibody at dilution of 1:100 (40x lens).