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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
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- Product number
- GTX12193 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX12193, RRID:AB_372435
- Product name
- SIRT2 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Nuclear extract of HeLa (lanes 1-2) and lysate of Hct-116 (lanes 3-4) cells were separated on SDS-PAGE, blotted with Anti-Sir2 (GTX12193) and developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. Lanes 1,3: Antibody 1 μg/mL 2,4: Antibody 1 μg/mL + Sir2 immunizing peptide (human, 722-737)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Nuclear extract of NIH-3T3 cells were separated on SDS-PAGE, blotted with Anti-Sir2 (GTX12193) and developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. Antibody 0.25 μg/mL 2. Antibody 0.5 μg/mL 3. Negative control: without first antibody
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NIH3T3 cells were fixed and stained with 20 μg/mL Anti-Sir2 (GTX12193). The antibody was developed using Goat Anti-Rabbit IgG, Atto 488 conjμgate. Nuclear staining with DAPI.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HeLa cells were fixed and stained with 20 μg/mL Anti-Sir2 (GTX12193). The antibody was developed using Goat Anti-Rabbit IgG, Atto 488 conjμgate. Nuclear staining with DAPI.
