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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00010110-M17 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00010110-M17, RRID:AB_894248
- Product name
- SGK2 monoclonal antibody (M17), clone 2F6
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant SGK2.
- Antigen sequence
GVEPEDTTSTFCGTPEYLAPEVLRKEPYDRAVDWW
CLGAVLYEMLHGLPPFYSQDVSQMYENILHQPLQI
PGGRTVAACDLLQSLLHKDQRQRLGSKADFL- Isotype
- IgG
- Antibody clone number
- 2F6
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of SGK2 expression in transfected 293T cell line by SGK2 monoclonal antibody (M17), clone 2F6.Lane 1: SGK2 transfected lysate(41.2 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to SGK2 on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of SGK2 transfected lysate using anti-SGK2 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with SGK2 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
