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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA1-46161 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TIP47 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- In Western Blot analysis, a band is seen ~47 kDa (isoform B, containing the first 184 residues that isoform A is lacking).
- Concentration
- 1 mg/mL
Submitted references PI3Kδ coordinates transcriptional, chromatin, and metabolic changes to promote effector CD8(+) T cells at the expense of central memory.
Cannons JL, Villarino AV, Kapnick SM, Preite S, Shih HY, Gomez-Rodriguez J, Kaul Z, Shibata H, Reilley JM, Huang B, Handon R, McBain IT, Gossa S, Wu T, Su HC, McGavern DB, O'Shea JJ, McGuire PJ, Uzel G, Schwartzberg PL
Cell reports 2021 Oct 12;37(2):109804
Cell reports 2021 Oct 12;37(2):109804
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TIP47 in 3T3 L1 lysate. Sample was incubated in TIP47 polyclonal antibody (Product # PA1-46161).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TIP47 in 0.5 mg/mL NIH-3T3 lysate. Samples were incubated in TIP47 polyclonal antibody (Product # PA1-46161). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of TIP47 in U2OS cells. Samples were incubated in TIP47 polyclonal antibody (Product # PA1-46161) followed by DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 549 (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6. IL-15 differentiated Pik3cd E1020K/+ OT-1 CD8 + T cells resemble effector cells (A-E) OT-1 cells activated with OVA 257-264 for 3 days and then cultured with either IL-2 or IL-15 to generate effector-like or memory-like cells, respectively (see Figure S6A ). (A) GzmB, CD69, and CD62L (n=3, representative flow plots). (B-E) RNA-seq analysis. (B) Principal-component analysis (PCA) of the two most variant TPM data components (WT cells, circles; Pik3cd E1020K/+ , triangles). (C) Heatmap of TPM values and Euclidian clustering for DEGs (rows) and experimental groups (columns). (D and E) Enrichment across 10 row clusters (C) of KEGG pathways (D) or curated CD8 + T cell gene sets (E) from http://www.gsea-msigdb.org/gsea/index.jsp . (F and G) OCR under basal conditions and in response to mitochondrial inhibitors oligomycin, FCCP, and antimycin A plus rotenone and calculated as the percent SRC in cells differentiated in IL-2 (F) and IL-15 (G) (representative data, n = 4). (H) LAL, Tip47, and AKT from IL-2 or IL-15 cultured cells (n = 2). Graphs (F and G) show mean +- SEM, percent SRC p < 0.0001. See Figure S7 and Table S2 .
