PA5-20273

antibody from Invitrogen Antibodies

Targeting: PLIN3 M6PRBP1, PP17, TIP47

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-20273

Provider

Invitrogen Antibodies

Product name

TIP47 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

A suggested positive control is EL4 cell lysate.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of EL4 cell lysate using a TIP47 polyclonal antibody (Product # PA5-20273) at (A) 0.5 and (B) 1 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: TIP47 Polyclonal Antibody (Product # PA5-20273) (1 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot Validation in Mouse Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: TIP47 Polyclonal Antibody (Product # PA5-20273) (1 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot Validation in Mouse EL4 Cells. Loading: 15 µg of lysates per lane. Antibodies: TIP47 Polyclonal Antibody (Product # PA5-20273) 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution. Lane 1: 1 µg/mL Lane 2: 2 µg/mL

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded rat liver tissue using TIP47 Polyclonal Antibody (Product # PA5-20273) at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.