14994-1-AP

antibody from Proteintech Group

Targeting: METTL1 C12orf1, TRM8, TRMT8

Provider product page for 14994-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [40]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [2]

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Product number: 14994-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#14994-1-AP, RRID:AB_2142013
Product name: METTL1 antibody
Antibody type: Polyclonal
Description: KD/KO validated METTL1 antibody (Cat. #14994-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

METTL1 protein structure - 14994-1-AP shown in red.

Submitted references Exosomes From Cancer-Associated Fibroblasts Promote Colorectal Cancer Progression and Glutamine Metabolism Through METTL1-Mediated m7G Modification of SLC1A5 mRNA.

Zhang S, Chen Z, Ma C, Hu J, Lin S, Gao J, Chen S
Journal of gastroenterology and hepatology 2026 May;41(5):1541-1555

METTL1-deficient mesenchymal stem cells protect against metabolic-associated fatty liver disease by increasing NAMPT secretion.

Du J, Zhang Y, Wang C, Wang Y, Zhang H, Zhao D, Lin J
Stem cells translational medicine 2026 Mar 23;15(4)

Circ_0003323 binds METTL1 to mediate KLK6 m7G methylation modification and promote the progression of colorectal cancer.

Zhang M, Li W, Ma J, Mu H, Zhao Y, Qi L, Liu D, Peng T
iScience 2026 Mar 20;29(3):114999

Dual Targeting of m(7)G tRNA Modification and Histone Acetylation using Carrier-Free Nano-Epidrugs to Evoke Osteosarcoma Chemosensitization.

Qi L, Zhang W, Shi C, Feng C, Liu Z, Li Z, Fang F, Chen X
Advanced materials (Deerfield Beach, Fla.) 2026 Jan;38(4):e05951

H3K18la-driven methyltransferase 1-mediated upregulation of NeuroD1 m7G modification in promoting ferroptosis resistance in rheumatoid arthritis synovial fibroblasts.

Xu Y, Liu W, Zai Z, Qian X, Hu W, Peng X, Chen F
International journal of biological macromolecules 2026 Jan;335(Pt 1):149196

METTL1 interacts with XPO5 to modulate pre-miRNA export.

Cao Z, Chen X, Sun Y, Wang Y
Nucleic acids research 2026 Jan 22;54(3)

Cancer-associated fibroblasts promote osimertinib resistance in non-small cell lung cancer cells via METTL1-mediated NET1 m(7)G modification.

Qian Y, Gong Z, Jia Y, Zhang Q, Cao L, Li B, Zhang J, Wang M, Wu X, Xu K
Cell death & disease 2026 Feb 21;17(1)

METTL1 drives glioma progression by promoting N7-methylguanosine (m7G) modification of glycolysis-related enzyme PGK1.

Fan B, Wang Y, Qin X, Yan Z, Du W
Pathology, research and practice 2025 Oct;274:156174

METTL1 Enhances RRP9 mRNA Stability Through m7G Modification to Drive Colorectal Tumorigenesis.

Li N, Jing Y, Xu L, Wang M
Molecular carcinogenesis 2025 May;64(5):858-869

A methyltransferase-independent role for METTL1 in tRNA aminoacylation and oncogenic transformation.

Ali RH, Orellana EA, Lee SH, Chae YC, Chen Y, Clauwaert J, Kennedy AL, Gutierrez AE, Papke DJ, Valenzuela M, Silverman B, Falzetta A, Ficarro SB, Marto JA, Fletcher CDM, Perez-Atayde A, Alcindor T, Shimamura A, Prensner JR, Gregory RI, Gutierrez A
Molecular cell 2025 Mar 6;85(5):948-961.e11

Construction of a diagnostic model utilizing m7G regulatory factors for the characterization of diabetic nephropathy and the immune microenvironment.

Zhong J, Xu P, Li X, Wang M, Chen X, Liang H, Chen Z, Yuan J, Xiao Y
Scientific reports 2025 Mar 17;15(1):9208

Microbial metabolite 5-formamidoimidazole-4-carboxamide ribotide targets METTL1 to inhibit m7G modification of BRCA1 mRNA to inhibit high-grade serous ovarian cancer.

Chen L, He S
Molecular medicine (Cambridge, Mass.) 2025 Dec 22;32(1):8

miR-193a-5p-mediated Inhibition of the METTL1/COX-2 axis is critical for Astragalin-induced apoptosis in cervical cancer.

Lee Y, Park SY, Shim BS, Kim B, Kim SH
Scientific reports 2025 Dec 13;16(1):2463

METTL1-driven epitranscriptomic enhancement of TXNDC12 boosts c-Myc stability through USP5 in HNSCC.

Mai Z, Zheng J, Lu Y, Lin P, Lin Y, Zheng Y, Chen X, Guo B, Cui L, Zhao X
Experimental & molecular medicine 2025 Aug;57(8):1743-1758

Rosmarinic Acid Alleviates Radiation-Induced Pulmonary Fibrosis by Downregulating the tRNA N7-Methylguanosine Modification-Regulated Fibroblast-to-Myofibroblast Transition Through the Exosome Pathway.

Zhang T, Mi J, Qin X, Ouyang Z, Wang Y, Li Z, He S, Hu K, Wang R, Huang W
Journal of inflammation research 2024;17:5567-5586

Metabolic reprogramming driven by METTL1-mediated tRNA m7G modification promotes acquired anlotinib resistance in oral squamous cell carcinoma.

Chen J, Zhou Q, Li S, Ling R, Zhao Y, Chen D, Wang A, Cao Y
Translational research : the journal of laboratory and clinical medicine 2024 Jun;268:28-39

WDR4 promotes HCC pathogenesis through N(7)-methylguanosine by regulating and interacting with METTL1.

Dong R, Wang C, Tang B, Cheng Y, Peng X, Yang X, Ni B, Li J
Cellular signalling 2024 Jun;118:111145

M(7)G modification of FTH1 and pri-miR-26a regulates ferroptosis and chemotherapy resistance in osteosarcoma.

He M, Wang Y, Xie J, Pu J, Shen Z, Wang A, Li T, Wang T, Li G, Liu Y, Mei Z, Ren Z, Wang W, Liu X, Hong J, Liu Q, Lei H, He X, Du W, Yuan Y, Yang L
Oncogene 2024 Jan;43(5):341-353

METTL1 mediated tRNA m(7)G modification promotes leukaemogenesis of AML via tRNA regulated translational control.

Zhao P, Xia L, Chen D, Xu W, Guo H, Xu Y, Yan B, Wu X, Li Y, Zhang Y, Zhang X
Experimental hematology & oncology 2024 Jan 24;13(1):8

Stixová L, Tichý V, Bártová E
Heliyon 2024 Feb 29;10(4):e25599

Targeting m7G-enriched circKDM1A prevents colorectal cancer progression.

Sun Z, Xu Y, Si C, Wu X, Guo Y, Chen C, Wang C
Molecular cancer 2024 Aug 30;23(1):179

IGF2BP3 promotes mRNA degradation through internal m(7)G modification.

Liu C, Dou X, Zhao Y, Zhang L, Zhang L, Dai Q, Liu J, Wu T, Xiao Y, He C
Nature communications 2024 Aug 28;15(1):7421

Mettl1-mediated internal m(7)G methylation of Sptbn2 mRNA elicits neurogenesis and anti-alzheimer's disease.

Li Q, Liu H, Li L, Guo H, Xie Z, Kong X, Xu J, Zhang J, Chen Y, Zhang Z, Liu J, Xuan A
Cell & bioscience 2023 Oct 1;13(1):183

METTL1/WDR4-mediated tRNA m(7)G modification and mRNA translation control promote oncogenesis and doxorubicin resistance.

Wang Z, Yu P, Zou Y, Ma J, Han H, Wei W, Yang C, Zheng S, Guo S, Wang J, Liu L, Lin S
Oncogene 2023 Jun;42(23):1900-1912

QKI shuttles internal m(7)G-modified transcripts into stress granules and modulates mRNA metabolism.

Zhao Z, Qing Y, Dong L, Han L, Wu D, Li Y, Li W, Xue J, Zhou K, Sun M, Tan B, Chen Z, Shen C, Gao L, Small A, Wang K, Leung K, Zhang Z, Qin X, Deng X, Xia Q, Su R, Chen J
Cell 2023 Jul 20;186(15):3208-3226.e27

Structural basis of regulated m(7)G tRNA modification by METTL1-WDR4.

Li J, Wang L, Hahn Q, Nowak RP, Viennet T, Orellana EA, Roy Burman SS, Yue H, Hunkeler M, Fontana P, Wu H, Arthanari H, Fischer ES, Gregory RI
Nature 2023 Jan;613(7943):391-397

A Comprehensive Analysis of METTL1 to Immunity and Stemness in Pan-Cancer.

Gao Z, Xu J, Zhang Z, Fan Y, Xue H, Guo X, Deng L, Wang S, Zhao R, Zhang P, Li G
Frontiers in immunology 2022;13:795240

METTL1 promotes neuroblastoma development through m(7)G tRNA modification and selective oncogenic gene translation.

Huang Y, Ma J, Yang C, Wei P, Yang M, Han H, Chen HD, Yue T, Xiao S, Chen X, Li Z, Tang Y, Luo J, Lin S, Huang L
Biomarker research 2022 Sep 7;10(1):68

METTL1 drives tumor progression of bladder cancer via degrading ATF3 mRNA in an m(7)G-modified miR-760-dependent manner.

Xie H, Wang M, Yu H, Wang H, Ding L, Wang R, Luo W, Lu Z, Zheng Q, Ren L, Zhou Z, Su W, Xia L, Li G
Cell death discovery 2022 Nov 17;8(1):458

Aberrant translation regulated by METTL1/WDR4-mediated tRNA N7-methylguanosine modification drives head and neck squamous cell carcinoma progression.

Chen J, Li K, Chen J, Wang X, Ling R, Cheng M, Chen Z, Chen F, He Q, Li S, Zhang C, Jiang Y, Chen Q, Wang A, Chen D
Cancer communications (London, England) 2022 Mar;42(3):223-244

N(7)-methylguanosine tRNA modification promotes esophageal squamous cell carcinoma tumorigenesis via the RPTOR/ULK1/autophagy axis.

Han H, Yang C, Ma J, Zhang S, Zheng S, Ling R, Sun K, Guo S, Huang B, Liang Y, Wang L, Chen S, Wang Z, Wei W, Huang Y, Peng H, Jiang YZ, Choe J, Lin S
Nature communications 2022 Mar 18;13(1):1478

SMYD3 associates with the NuRD (MTA1/2) complex to regulate transcription and promote proliferation and invasiveness in hepatocellular carcinoma cells.

Yang Y, Qiu R, Zhao S, Shen L, Tang B, Weng Q, Xu Z, Zheng L, Chen W, Shu G, Wang Y, Zhao Z, Chen M, Ji J
BMC biology 2022 Dec 27;20(1):294

Prognostic role of METTL1 in glioma.

Li L, Yang Y, Wang Z, Xu C, Huang J, Li G
Cancer cell international 2021 Nov 27;21(1):633

METTL1/WDR4-mediated m(7)G tRNA modifications and m(7)G codon usage promote mRNA translation and lung cancer progression.

Ma J, Han H, Huang Y, Yang C, Zheng S, Cai T, Bi J, Huang X, Liu R, Huang L, Luo Y, Li W, Lin S
Molecular therapy : the journal of the American Society of Gene Therapy 2021 Dec 1;29(12):3422-3435

N(7)-Methylguanosine tRNA modification enhances oncogenic mRNA translation and promotes intrahepatic cholangiocarcinoma progression.

Dai Z, Liu H, Liao J, Huang C, Ren X, Zhu W, Zhu S, Peng B, Li S, Lai J, Liang L, Xu L, Peng S, Lin S, Kuang M
Molecular cell 2021 Aug 19;81(16):3339-3355.e8

METTL1-mediated m(7)G modification of Arg-TCT tRNA drives oncogenic transformation.

Orellana EA, Liu Q, Yankova E, Pirouz M, De Braekeleer E, Zhang W, Lim J, Aspris D, Sendinc E, Garyfallos DA, Gu M, Ali R, Gutierrez A, Mikutis S, Bernardes GJL, Fischer ES, Bradley A, Vassiliou GS, Slack FJ, Tzelepis K, Gregory RI
Molecular cell 2021 Aug 19;81(16):3323-3338.e14

Methyltransferase-like 1 regulates lung adenocarcinoma A549 cell proliferation and autophagy via the AKT/mTORC1 signaling pathway.

Wang C, Wang W, Han X, Du L, Li A, Huang G
Oncology letters 2021 Apr;21(4):330

Dynamic methylome of internal mRNA N(7)-methylguanosine and its regulatory role in translation.

Malbec L, Zhang T, Chen YS, Zhang Y, Sun BF, Shi BY, Zhao YL, Yang Y, Yang YG
Cell research 2019 Nov;29(11):927-941

Mettl1/Wdr4-Mediated m(7)G tRNA Methylome Is Required for Normal mRNA Translation and Embryonic Stem Cell Self-Renewal and Differentiation.

Lin S, Liu Q, Lelyveld VS, Choe J, Szostak JW, Gregory RI
Molecular cell 2018 Jul 19;71(2):244-255.e5

tRNA modifying enzymes, NSUN2 and METTL1, determine sensitivity to 5-fluorouracil in HeLa cells.

Okamoto M, Fujiwara M, Hori M, Okada K, Yazama F, Konishi H, Xiao Y, Qi G, Shimamoto F, Ota T, Temme A, Tatsuka M
PLoS genetics 2014 Sep;10(9):e1004639

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: A2780 cells were subjected to SDS PAGE followed by western blot with 14994-1-AP(METTL1 antibody) at dilution of 1:500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human gliomas using 14994-1-AP(METTL1 antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The METTL1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human METTL1. This antibody recognizes human, mouse, rat antigen. The METTL1 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Appendix: 14994-1-AP.pdf

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