66039-1-Ig

antibody from Proteintech Group

Targeting: CPT1A CPT1, CPT1-L, L-CPT1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

66039-1-Ig

Provider

Proteintech Group

Proper citation

Proteintech Cat#66039-1-Ig, RRID:AB_11041710

Product name

CPT1A antibody

Antibody type

Monoclonal

Description

KD/KO validated CPT1A antibody (Cat. #66039-1-Ig) is a mouse monoclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

5D8G9

Vial size

20ul, 150ul

References

Submitted references

Inhibiting TRIM21 Neddylation Rejuvenates Oocyte Quality in PCOS by Regulating Ubiquitination of CPT1A.

Na X, Liu J, Tan Y, Meng L, Guo C, Zuo N, Dai W, Cong R, Zhang B, Shi W, Hu J, Liang J, Wei S, Zhao Z, Chen J, Qiao X, Li D
Research (Washington, D.C.) 2026;9:1223

---

Dynamic and differential renal cortical cell-specific mitochondrial metabolism in response to fasting.

Feola K, Venable AH, Rasouli M, Do J, McCoy T, Llamas CB, Straus D, Russell JC, Mishra P, Najafian B, Huen SC
American journal of physiology. Renal physiology 2026 Feb 1;330(2):F210-F222

---

African swine fever virus hijacks lipolysis induced by chaperone-mediated autophagy to upregulate fatty acid β-oxidation and promote viral replication.

Yang X, Xiong X, Liu H, Wen X, Shi X, Ma H, Wang R, Cao W, Yang F, Ru Y, Tian H, He J, Guo J, Han S, Zhu Z, Zheng H
mBio 2026 Apr 8;17(4):e0336825

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Vitamin K(2) emerges as the key mediator: Cetobacterium somerae ZNN-1 increases muscle protein deposition and improves liver health in Nile tilapia (Oreochromis niloticus).

Zhou N, Liu J, Zhang X, Xiao G, Zhang M
Journal of animal science and biotechnology 2026 Apr 11;17(1)

---

PEDF Overexpression Ameliorates Cardiac Lipotoxicity in Diabetic Cardiomyopathy via Regulation of Energy Metabolism.

Mao T, Wang Y
Diabetes, metabolic syndrome and obesity : targets and therapy 2025;18:217-231

---

Autophagy-driven lipid regulation by an herbal decoction alleviates cardiac lipotoxicity in severe acute pancreatitis.

Yang Y, Hu Q, Kang H, Xie L, Hu Y, Li J, Zhao X, Zhu L, Tang W, Liu J, Lyon CJ, Wan M
Theranostics 2025;15(17):8822-8839

---

CPT1A mediated preservation of mitochondrial inhibits pyroptosis in pancreatic acinar cells.

Liu Y, Liu Y, Yu X, Tian S, Li X, Gao Y, Bao X, Wu X, Zhang B, Huang W
Frontiers in cell and developmental biology 2025;13:1577669

---

STING inhibits the progression of esophageal squamous cell carcinoma by suppressing CPT1A-mediated fatty acid β-oxidation.

Zhang L, Zhu LJ, Zhao Y, Lei XY, Wu DH, He KY, Liu MJ, Yang JY, Guo JR, Jiang ZH, Yan ZH, Xu ZX, Jian YP
Acta pharmacologica Sinica 2025 Oct;46(10):2793-2807

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Microplastics promote chemoresistance by mediating lipid metabolism and suppressing pyroptosis in colorectal cancer.

Hu D, Liu H, Guo Y, Zhang H, Qiu M, Yang Z, Hao J, Jiang Z, Gao M, Zhang X, Zhang M
Apoptosis : an international journal on programmed cell death 2025 Oct;30(9-10):2287-2300

---

AMPK Signaling Pathway Regulates Tendon Regeneration via Fatty Acid Metabolism.

Wu T, Zhu L, Yu M, Cai X, Chen L, Zhang H, Wu X, Ding C, Liu H, Zhang S, Li C, Shi X, Wang Y, Liu Y
Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2025 May;43(5):1012-1023

---

Multi-omics joint analysis: Pachymic acid ameliorated non-alcoholic fatty liver disease by regulating gut microbiota.

Ren G, Lin Y, Fu Y, Liu F, Wang R, Zhang C, Qiu J, Chen L, Dou X
Food research international (Ottawa, Ont.) 2025 May;209:116178

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Effects of polystyrene microparticles exposures on spermatogenic cell differentiation and reproductive endpoints in male mice.

Shen R, Xia P, Guo Y, Ji P, Yuan X, Wang L, Shuang S, Zhou L, Tong R, Zhang L, Liu D, Wang D
Environmental pollution (Barking, Essex : 1987) 2025 May 15;373:126200

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SLC31A1 promotes chemoresistance through inducing CPT1A-mediated fatty acid oxidation in ER-positive breast cancer.

Li X, Ge J, Wan M, Feng T, Li X, Zhang H, Wang Z, Gao Y, Chen M, Pan F
Neoplasia (New York, N.Y.) 2025 Mar;61:101125

---

SIRT5 mediated succinylation of SUCLA2 regulates TCA cycle dysfunction and mitochondrial damage in pancreatic acinar cells in acute pancreatitis.

Liu W, Wang X, Xu D, Gong F, Pei L, Yang S, Zhao S, Zheng X, Li R, Yang Z, Fei J, Mao E, Chen E, Chen Y
Biochimica et biophysica acta. Molecular basis of disease 2025 Mar;1871(3):167613

---

Increasing cisplatin exposure promotes small-cell lung cancer transformation after a shift from glucose metabolism to fatty acid metabolism.

Zhao QY, Liu WJ, Wang JG, Li H, Lv JL, Wang Y, Wang C
Journal of cancer research and clinical oncology 2025 Mar 28;151(3):126

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Cardioprotective effects of the jiming formula on myocardial metabolism in Mice with myocardial infarction via the AMPK/SIRT1/PGC-1α pathway.

Fan X, Wei X, Wang W, Chai W, Xiao J, Han J, Li F, Guo S
Phytomedicine : international journal of phytotherapy and phytopharmacology 2025 Jun;141:156727

---

Stromal lipid species dictate melanoma metastasis and tropism.

Gurung S, Budden T, Mallela K, Jenkins B, von Kriegsheim A, Manrique E, Millán-Esteban D, Romero-Camarero I, Amaral F, Craig S, Durao P, Pozniak J, Stennett L, Smith D, Ashton G, Baker A, Zeng K, Fruhwirth G, Sanz-Moreno V, Marques J, Koulman A, Marine JC, Somervaille TCP, Motta L, Gaudy-Marqueste C, Nagore E, Virós A
Cancer cell 2025 Jun 9;43(6):1108-1124.e11

---

Measuring renal cortical cell-specific mitochondrial metabolism.

Feola K, Venable AH, Rasouli M, Do J, McCoy T, Llamas CB, Straus D, Mishra P, Najafian B, Huen SC
bioRxiv : the preprint server for biology 2025 Jun 12;

---

Hericium erinaceus Protein Alleviates High-Fat Diet-Induced Hepatic Lipid Accumulation and Oxidative Stress In Vivo.

Lu H, Yang S, Li W, Zheng B, Zeng S, Chen H
Foods (Basel, Switzerland) 2025 Jan 31;14(3)

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CPT1A mediates succinylation of LDHA at K318 site promoteing metabolic reprogramming in NK/T-cell lymphoma nasal type.

Tian H, Ge Y, Yu J, Chen X, Wang H, Cai X, Shan Z, Zuo L, Liu Y
Cell biology and toxicology 2025 Feb 11;41(1):42

---

SIRT5 regulates granulosa cell proliferation and apoptosis in polycystic ovarian syndrome via desuccinylation of GLI1.

Zhang Y, He F, Cai N, Chen G, Wang Y, Bai W, Guo P
Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology 2025 Dec;41(1):2515516

---

SIRT5 regulates fatty acid oxidation and mitochondrial oxidative stress to exacerbate airway inflammation in experimental asthma.

Wu J, Wang Y, Zhang Q, Lan Y, Chen M, Wang J, Zhao X, Chen Y, Peng X, Zhao W, Zhao H
Cellular signalling 2025 Dec;136:112149

---

CD36-mediated fatty acid metabolic reprogramming activates synovial fibroblasts and promotes rheumatoid arthritis progression.

Hao J, Zheng J, Liu C, Lu J, Tan M, Li J, Mao J, Yu Y, Wang H, Zhang Y, Shen H
International immunopharmacology 2025 Dec 10;167:115594

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Ginkgolide B attenuates hyperlipidemia by restoring sphingolipid homeostasis and activating PPARα and Nrf2 pathways.

Liu Y, Wang F, Xu H, Wang H, Lu M, Cheng L
Scientific reports 2025 Aug 6;15(1):28774

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Elevated levels of Letm1 drives mitochondrial dysfunction and cardiomyocyte stress-mediated apoptosis in cultured cardiomyocytes.

Deshpande A, Weirauch L, Baral TK, Steier M, Borlepawar A, Kumari M, Kilian LS, Richter K, Hammer E, Frank D, Schmidt C, Frey N, Rangrez AY
Cell communication and signaling : CCS 2025 Aug 23;23(1):378

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PRMT1-mediated metabolic reprogramming promotes leukemogenesis.

Su H, Sun Y, Guo H, Sun CW, Chen Q, Liu S, Li A, Gao M, Zhao R, Raffel G, Jin J, Qu CK, Yu M, Klug CA, Zheng GY, Ballinger S, Kutny M, Zheng LX, Chong Z, Senevirathne C, Gross S, Chen Y, Luo M, Zhao X
eLife 2025 Aug 13;14

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Qizhu Rougan Granules suppress liver fibrosis by inhibiting the expression of the P2Y14 receptor on hepatic stellate cells.

Tao Y, Niu Q, Yao Y, Wang K, Dong H, Zhao X, Zeng Z, Li H
Frontiers in pharmacology 2024;15:1528100

---

Bushen Huoxue formula attenuates lipid accumulation evoking excessive autophagy in premature ovarian insufficiency rats and palmitic acid-challenged KGN cells by modulating lipid metabolism.

Li T, Wei Y, Jiao B, Hao R, Zhou B, Bian X, Wang P, Zhou Y, Sun X, Zhang J
Frontiers in pharmacology 2024;15:1425844

---

Cpt1a Drives primed-to-naïve pluripotency transition through lipid remodeling.

Ma Z, Huang X, Kuang J, Wang Q, Qin Y, Huang T, Liang Z, Li W, Fu Y, Li P, Fan Y, Zhai Z, Wang X, Ming J, Zhao C, Wang B, Pei D
Communications biology 2024 Sep 30;7(1):1223

---

Increase in GPIHBP1 expression in advanced stage colorectal cancer indicates poor immune surveillance.

Gao M, Liao L, Lin Z, Hu X, Jia L, Gong W, Jia X
Translational cancer research 2024 Jun 30;13(6):2691-2703

---

Bruceine A alleviates alcoholic liver disease by inhibiting AIM2 inflammasome activation via activating FXR.

Li L, Xu S, Wang W, Li X, Wang H, Yang Q, Wang C, Gu J, Luo H, Meng Q
Phytomedicine : international journal of phytotherapy and phytopharmacology 2024 Jul 25;130:155693

---

Farnesoid X receptor overexpression prevents hepatic steatosis through inhibiting AIM2 inflammasome activation in nonalcoholic fatty liver disease.

Xu S, Kong L, Li L, Wang C, Gu J, Luo H, Meng Q
Biochimica et biophysica acta. Molecular basis of disease 2024 Feb;1870(2):166930

---

FXR overexpression prevents hepatic steatosis through inhibiting AIM2 inflammasome activation in alcoholic liver disease.

Li L, Kong L, Xu S, Wang C, Gu J, Luo H, Meng Q
Hepatology international 2024 Feb;18(1):188-205

---

Ganoderma lucidum Spore Powder Alleviates Metabolic-Associated Fatty Liver Disease by Improving Lipid Accumulation and Oxidative Stress via Autophagy.

Zhang Y, Zhou J, Yang L, Xiao H, Liu D, Kang X
Antioxidants (Basel, Switzerland) 2024 Dec 9;13(12)

---

Impaired intestinal FXR signaling is involved in aberrant stem cell function leading to intestinal failure-associated liver disease in pediatric patients with short bowel syndrome.

Zhao Y, Wang Y, Jiang L, Cai W, Yan J
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2024 Aug 15;38(15):e23847

---

Regulation of astrocyte metabolism by mitochondrial translocator protein 18kDa.

Firth W, Robb JL, Stewart D, Pye KR, Bamford R, Oguro-Ando A, Beall C, Ellacott KL
bioRxiv : the preprint server for biology 2023 Oct 2;

---

Angelica sinensis polysaccharide ameliorates nonalcoholic fatty liver disease via restoring estrogen-related receptor α expression in liver.

Luo L, Zhang H, Chen W, Zheng Z, He Z, Wang H, Wang K, Zhang Y
Phytotherapy research : PTR 2023 Nov;37(11):5407-5417

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L-carnitine alleviates cardiac microvascular dysfunction in diabetic cardiomyopathy by enhancing PINK1-Parkin-dependent mitophagy through the CPT1a-PHB2-PARL pathways.

Li S, Liu M, Chen J, Chen Y, Yin M, Zhou Y, Li Q, Xu F, Li Y, Yan X, Xia Y, Chen A, Lu D, Li C, Shen L, Chen Z, Qian J, Ge J
Acta physiologica (Oxford, England) 2023 Jul;238(3):e13975

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Impaired FXR-CPT1a signaling contributes to parenteral nutrition-induced villus atrophy in short-bowel syndrome.

Zhao Y, Feng H, Wang Y, Jiang L, Yan J, Cai W
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2023 Jan;37(1):e22713

---

Altered Peroxisome Proliferator-Activated Receptor Alpha Signaling in Variably Diseased Peripheral Arterial Segments.

Engel C, Meade R, Harroun N, Penrose A, Shafqat M, Jin X, DeSilva G, Semenkovich C, Zayed M
Frontiers in cardiovascular medicine 2022;9:834199

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Berberrubine, a Main Metabolite of Berberine, Alleviates Non-Alcoholic Fatty Liver Disease via Modulating Glucose and Lipid Metabolism and Restoring Gut Microbiota.

Yang S, Cao S, Li C, Zhang J, Liu C, Qiu F, Kang N
Frontiers in pharmacology 2022;13:913378

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The serum acylcarnitines profile in epileptic children treated with valproic acid and the protective roles of peroxisome proliferator-activated receptor a activation in valproic acid-induced liver injury.

Ma Y, Wang M, Guo S, Li T, Liu X, Zhao L
Frontiers in pharmacology 2022;13:1048728

---

Lipidomics reveals the potential mechanism of honokiol against adenine-induced chronic kidney disease.

Liu X, Gao L, Huang X, Deng R, Wei X, Lu J, Li S
Frontiers in pharmacology 2022;13:1019629

---

Hawk tea prevents high-fat diet-induced obesity in mice by activating the AMPK/ACC/SREBP1c signaling pathways and regulating the gut microbiota.

Tao W, Cao W, Yu B, Chen H, Gong R, Luorong Q, Luo J, Yao L, Zhang D
Food & function 2022 Jun 6;13(11):6056-6071

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PD-1 signaling facilitates activation of lymphoid tissue inducer cells by restraining fatty acid oxidation.

Wu D, Hu L, Han M, Deng Y, Zhang Y, Ren G, Zhao X, Li Z, Li P, Zhang Y, Chen S, Li J, Shi Y, Xue J, Wang P, Zhong C
Nature metabolism 2022 Jul;4(7):867-882

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MicroRNA-377-3p inhibits hepatocellular carcinoma growth and metastasis through negative regulation of CPT1C-mediated fatty acid oxidation.

Zhang T, Zhang Y, Liu J, Ma Y, Ye Q, Yan X, Ding L
Cancer & metabolism 2022 Jan 20;10(1):2

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The lncRNA MIAT regulates CPT-1a mediated cardiac hypertrophy through m(6)A RNA methylation reading protein Ythdf2.

Yang Y, Mbikyo MB, Zhang J, Zhang Y, Zhang N, Li Z
Cell death discovery 2022 Apr 5;8(1):167

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Multi-Omics Analysis of Fatty Acid Metabolism in Thyroid Carcinoma.

Lu J, Zhang Y, Sun M, Ding C, Zhang L, Kong Y, Cai M, Miccoli P, Ma C, Yue X
Frontiers in oncology 2021;11:737127

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CEPT1-Mediated Phospholipogenesis Regulates Endothelial Cell Function and Ischemia-Induced Angiogenesis Through PPARα.

Zayed MA, Jin X, Yang C, Belaygorod L, Engel C, Desai K, Harroun N, Saffaf O, Patterson BW, Hsu FF, Semenkovich CF
Diabetes 2021 Feb;70(2):549-561

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Etomoxir regulates the differentiation of male germ cells by specifically reducing H3K27ac level.

Xu Y, Xie J
BMC developmental biology 2021 Feb 1;21(1):5

---

Curcumin-enhanced antitumor effects of sorafenib via regulating the metabolism and tumor microenvironment.

Man S, Yao J, Lv P, Liu Y, Yang L, Ma L
Food & function 2020 Jul 22;11(7):6422-6432

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Metabolomics reveal mitochondrial and fatty acid metabolism disorders that contribute to the development of DKD in T2DM patients.

Li L, Wang C, Yang H, Liu S, Lu Y, Fu P, Liu J
Molecular bioSystems 2017 Oct 24;13(11):2392-2400

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Circadian and Dopaminergic Regulation of Fatty Acid Oxidation Pathway Genes in Retina and Photoreceptor Cells.

Vancura P, Wolloscheck T, Baba K, Tosini G, Iuvone PM, Spessert R
PloS one 2016;11(10):e0164665

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

L02 cells were subjected to SDS PAGE followed by western blot with 66039-1-Ig(CPT1A antibody) at dilution of 1:500

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-CPT1A (IP:66039-1-Ig, 4ug; Detection:66039-1-Ig 1:500) with MCF-7 cells lysate 1200ug.

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human heart using 66039-1-Ig(CPT1A antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The CPT1A antibody from Proteintech is a mouse monoclonal antibody to a fusion protein of human CPT1A. This antibody recognizes human antigen. The CPT1A antibody has been validated for the following applications: ELISA, WB, IHC, IP analysis.

Appendix

66039-1-Ig.pdf

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