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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- PA5-29995 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CPT1A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, HeLa, HepG2.
- Concentration
- 1 mg/mL
Submitted references Combined Inhibition of the Renin-Angiotensin System and Neprilysin Positively Influences Complex Mitochondrial Adaptations in Progressive Experimental Heart Failure.
Grois L, Hupf J, Reinders J, Schröder J, Dietl A, Schmid PM, Jungbauer C, Resch M, Maier LS, Luchner A, Birner C
PloS one 2017;12(1):e0169743
PloS one 2017;12(1):e0169743
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CPT1A using 30 µg of HepG2 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a CPT1A polyclonal antibody (Product # PA5-29995) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CPT1A Polyclonal Antibody (Product # PA5-29995). Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CPT1A Polyclonal Antibody (Product # PA5-29995) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CPT1A Polyclonal Antibody detects CPT1A protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. CPT1A stained by CPT1A Polyclonal Antibody (Product # PA5-29995) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded MDA-MB157 xenograft, using CPT1A (Product # PA5-29995) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
