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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-27201 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ACADM Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Molt-4, Neuro2A, GL261, C8D30, PC-12.
- Concentration
- 0.55 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ACADM Polyclonal Antibody (Product # PA5-27201). Sample (30 µg of whole cell lysate). A: MOLT4SDS PAGE. Z. 7.5% SDS PAGE. ACADM Polyclonal Antibody (Product # PA5-27201) diluted at 1:2,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ACADM Polyclonal Antibody detects ACADM protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract. B. 30 µg GL261 whole cell lysate/extract. C. 30 µg C8D30 whole cell lysate/extract.10 % SDS-PAGE. ACADM Polyclonal Antibody (Product # PA5-27201) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ACADM Polyclonal Antibody detects ACADM protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract.10 % SDS-PAGE. ACADM Polyclonal Antibody (Product # PA5-27201) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of ACADM was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: ACADM Polyclonal Antibody (Product # PA5-27201) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ACADM Polyclonal Antibody detects ACADM protein at mitochondria on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. ACADM Polyclonal Antibody (Product # PA5-27201) dilution: 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
