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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-27201 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ACADM Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Molt-4, Neuro2A, GL261, C8D30, PC-12. Predicted reactivity: Mouse (89%), Rat (88%), Zebrafish (87%), Xenopus laevis (87%), Pig (91%), Chimpanzee (99%), Bovine (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.55 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of ACADM was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: ACADM Polyclonal Antibody (Product # PA5-27201) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of ACADM was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: ACADM Polyclonal Antibody (Product # PA5-27201) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ACADM Polyclonal Antibody detects ACADM protein at mitochondria on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. ACADM Polyclonal Antibody (Product # PA5-27201) dilution: 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
