16754-1-AP

antibody from Proteintech Group

Targeting: PCK1 PEPCK-C

Provider product page for 16754-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [66]
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Validations
Western blot [1]

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Product number: 16754-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#16754-1-AP, RRID:AB_2160031
Product name: PCK1 antibody
Antibody type: Polyclonal
Description: KD/KO validated PCK1 antibody (Cat. #16754-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

PCK1 protein structure - 16754-1-AP shown in red.

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Plata-Gómez AB, de Prado-Rivas L, Sanz A, Deleyto-Seldas N, García F, de la Calle Arregui C, Silva C, Caleiras E, Graña-Castro O, Piñeiro-Yáñez E, Krebs J, Leiva-Vega L, Muñoz J, Jain A, Sabio G, Efeyan A
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Okur MN, Sahbaz BD, Kimura R, Manor U, Patel J, Park JH, Andrade L, Puligilla C, Croteau DL, Bohr VA
Aging cell 2023 Sep;22(9):e13909

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International journal of biological macromolecules 2023 Sep 30;249:126039

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Lin Q, Guo Y, Li J, He S, Chen Y, Jin H
Marine drugs 2023 Sep 29;21(10)

Paternal cadmium exposure induces glucolipid metabolic reprogramming in offspring mice via PPAR signaling pathway.

Zeng L, Zhou J, Zhang Y, Wang X, Li Y, Song J, Shao J, Su P
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Cellular and molecular life sciences : CMLS 2023 May 30;80(6):168

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Lee RA, Chang M, Yiv N, Tsay A, Tian S, Li D, Poulard C, Stallcup MR, Pufall MA, Wang JC
Nature communications 2023 May 30;14(1):3143

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Li Z, Zeng M, Geng K, Lai D, Xu Z, Zhou W
Molecules (Basel, Switzerland) 2023 Mar 16;28(6)

Bavachin protects against diet-induced hepatic steatosis and obesity in mice.

Wei X, Lin L, Yuan QQ, Wang XY, Zhang Q, Zhang XM, Tang KC, Guo MY, Dong TY, Han W, Huang DK, Qi YL, Zhang M, Zhang HB
Acta pharmacologica Sinica 2023 Jul;44(7):1416-1428

A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy.

Southwell N, Primiano G, Nadkarni V, Attarwala N, Beattie E, Miller D, Alam S, Liparulo I, Shurubor YI, Valentino ML, Carelli V, Servidei S, Gross SS, Manfredi G, Chen Q, D'Aurelio M
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PCK1 activates oncogenic autophagy via down-regulation Serine phosphorylation of UBAP2L and antagonizes colorectal cancer growth.

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Cancer cell international 2023 Apr 16;23(1):68

G-protein coupled receptor 19 (GPR19) knockout mice display sex-dependent metabolic dysfunction.

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Empagliflozin Inhibits Hepatic Gluconeogenesis and Increases Glycogen Synthesis by AMPK/CREB/GSK3β Signalling Pathway.

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Wang Y, Yan S, Xiao B, Zuo S, Zhang Q, Chen G, Yu Y, Chen D, Liu Q, Liu Y, Shen Y, Yu Y
Diabetes 2018 Sep;67(9):1748-1760

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Qi X, Guo Y, Song Y, Yu C, Zhao L, Fang L, Kong D, Zhao J, Gao L
Diabetologia 2018 May;61(5):1180-1192

NaoXinTong Capsules inhibit the development of diabetic nephropathy in db/db mice.

Yang S, Liu M, Chen Y, Ma C, Liu L, Zhao B, Wang Y, Li X, Zhu Y, Gao X, Kong D, Duan Y, Han J, Yang X
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Catalpol ameliorates hepatic insulin resistance in type 2 diabetes through acting on AMPK/NOX4/PI3K/AKT pathway.

Yan J, Wang C, Jin Y, Meng Q, Liu Q, Liu Z, Liu K, Sun H
Pharmacological research 2018 Apr;130:466-480

Role of AMPD2 in impaired glucose tolerance induced by high fructose diet.

Hudoyo AW, Hirase T, Tandelillin A, Honda M, Shirai M, Cheng J, Morisaki H, Morisaki T
Molecular genetics and metabolism reports 2017 Dec;13:23-29

Novel homozygous PCK1 mutation causing cytosolic phosphoenolpyruvate carboxykinase deficiency presenting as childhood hypoglycemia, an abnormal pattern of urine metabolites and liver dysfunction.

Vieira P, Cameron J, Rahikkala E, Keski-Filppula R, Zhang LH, Santra S, Matthews A, Myllynen P, Nuutinen M, Moilanen JS, Rodenburg RJ, Rolfs A, Uusimaa J, van Karnebeek CDM
Molecular genetics and metabolism 2017 Apr;120(4):337-341

Cytosolic phosphoenolpyruvate carboxykinase deficiency presenting with acute liver failure following gastroenteritis.

Santra S, Cameron JM, Shyr C, Zhang L, Drögemöller B, Ross CJ, Wasserman WW, Wevers RA, Rodenburg RJ, Gupte G, Preece MA, van Karnebeek CD
Molecular genetics and metabolism 2016 May;118(1):21-7

Administration of Danhong Injection to diabetic db/db mice inhibits the development of diabetic retinopathy and nephropathy.

Liu M, Pan Q, Chen Y, Yang X, Zhao B, Jia L, Zhu Y, Zhang B, Gao X, Li X, Han J, Duan Y
Scientific reports 2015 Jun 10;5:11219

Betaine supplementation in maternal diet modulates the epigenetic regulation of hepatic gluconeogenic genes in neonatal piglets.

Cai D, Jia Y, Song H, Sui S, Lu J, Jiang Z, Zhao R
PloS one 2014;9(8):e105504

Circadian clock-dependent and -independent rhythmic proteomes implement distinct diurnal functions in mouse liver.

Mauvoisin D, Wang J, Jouffe C, Martin E, Atger F, Waridel P, Quadroni M, Gachon F, Naef F
Proceedings of the National Academy of Sciences of the United States of America 2014 Jan 7;111(1):167-72

Breed-dependent transcriptional regulation of phosphoenolpyruvate carboxylase, cytosolic form, expression in the liver of broiler chickens.

Guo F, Zhang Y, Su L, Ahmed AA, Ni Y, Zhao R
Poultry science 2013 Oct;92(10):2737-44

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The PCK1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human PCK1. This antibody recognizes human,mouse,rat antigen. The PCK1 antibody has been validated for the following applications: ELISA, WB analysis.
Appendix: 16754-1-AP.pdf

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