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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- F54453 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PEPCK1 Antibody / PCK1 / PEPC
- Antibody type
- Polyclonal
- Description
- This highly specific PEPCK1 antibody is suitable for use in Western blot/Immunofluorescence/Immunohistochemistry applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 0.08 ml, 0.4 ml
- Concentration
- In 1X PBS, pH 7.4, with 0.09% sodium azide
- Storage
- Aliquot the PEPCK1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human liver, 2) human kidney, 3) human NCI-H460, 4) mouse liver, 5) mouse kidney and 6) rat kidney lysate with PEPCK1 antibody. Predicted molecular weight: ~69 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human HepG2 cells with PEPCK1 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human kidney tissue with PEPCK1 antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.
