PA5-15364

antibody from Invitrogen Antibodies

Targeting: PCK1 PEPCK-C

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-15364

Provider

Invitrogen Antibodies

Product name

PCK1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

2.0 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Testosterone supplementation improves glucose homeostasis despite increasing hepatic insulin resistance in male mouse model of type 2 diabetes mellitus.

Pal M, Gupta S
Nutrition & diabetes 2016 Dec 12;6(12):e236

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PCK1 in formalin-fixed and paraffin-embedded human cancer tissue. Samples were incubated with PCK1 polyclonal antibody (Product # PA5-15364) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 ( a ) Schematic representation of HFD feeding and treatment duration in animals. ( b ) Reduced hepatic glucose output in Treated animals. ( b ) Fasting BGL of Control and Treated animals. The data were analyzed by t -test, data represent mean+-s.d., n =8, P 0.05; ( c - e ) PTT in Control and Treated animals after 4 ( c ), 16 ( d ) and 32 ( e ) weeks of treatment. The data were analyzed by t -test, data represent mean+-s.d., n =8, P 0.1. ( g - j ) Immonoblot analyses depict reduced PEPCK level, but increased insulin resistance in the liver of Treated animals. ( g ) Immunoblot and densitometry for PEPCK levels in the liver of Control and Treated animals. The data were analyzed by t -test, data represent mean+-s.d. of three independent experiments ( n =3), P