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LearnH00009572-M02
antibody from Abnova Corporation
Targeting: NR1D1
ear-1, hRev, Rev-ErbAalpha, REVERBA, REVERBalpha, THRA1, THRAL
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00009572-M02 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00009572-M02, RRID:AB_566129
- Product name
- NR1D1 monoclonal antibody (M02), clone 4F6
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant NR1D1.
- Antigen sequence
MTTLDSNNNTGGVITYIGSSGSSPSRTSPESLYSD
NSNGSFQSLTQGCPTYFPPSPTGSLTQDPARSFGS
IPPSLSDDGSPSSSSSSSSSSSSFYNGSPPGSLQV
AMEDSSRVSPSKSTSNITKLNGMVLLCKVCGDVAS
GFHYGVHACEGCKGFFRRSIQQNIQYKRCLKNENC
SIVRINRNRCQQCRFKKCLSVGMSRDAVRFGRIPK
REKQRMLAEMQSAMNLANNQLSSQCPLETSPTQHP
TPGPMGPSPPPAPVPSPLVGFSQFPQQLTPPRSPS
PEPTVEDVISQVARAHREIFTYAHDKLGSSPGNFN
ANHASGSPPATTPHRWENQGCPPAPNDNNTLAAQR
HNEALNGLRQAPSSYPPTWPPGPAHHSCHQSNSNG
HRLCPTHVYAAPEGKAPANSPRQGNSKNVLLACPM
NMYPHGRSGRTVQEIWEDFSMSFTPAVREVVEFAK
HIPGFRDLSQHDQVTLLKAGTFEVLMVRFASLFNV
KDQTVMFLSRTTYSLQELGAMGMGDLLSAMFDFSE
KLNSLALTEEELGLFTAVVLVSADRSGMENSASVE
QLQETLLRALRALVLKNRPLETSRFTKLLLKLPDL
RTLNNMHSEKLLSFRVDAQ- Isotype
- IgG
- Antibody clone number
- 4F6
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Ubiquitin ligase Siah2 regulates RevErbα degradation and the mammalian circadian clock.
Clock-controlled output gene Dbp is a regulator of Arnt/Hif-1β gene expression in pancreatic islet β-cells.
DeBruyne JP, Baggs JE, Sato TK, Hogenesch JB
Proceedings of the National Academy of Sciences of the United States of America 2015 Oct 6;112(40):12420-5
Proceedings of the National Academy of Sciences of the United States of America 2015 Oct 6;112(40):12420-5
Clock-controlled output gene Dbp is a regulator of Arnt/Hif-1β gene expression in pancreatic islet β-cells.
Nakabayashi H, Ohta Y, Yamamoto M, Susuki Y, Taguchi A, Tanabe K, Kondo M, Hatanaka M, Nagao Y, Tanizawa Y
Biochemical and biophysical research communications 2013 May 3;434(2):370-5
Biochemical and biophysical research communications 2013 May 3;434(2):370-5
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- NR1D1 monoclonal antibody (M02), clone 4F6. Western Blot analysis of NR1D1 expression in NIH/3T3 ( Cat # L018V1 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged NR1D1 is approximately 1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to NR1D1 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to NR1D1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
