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LearnH00009572-M02
antibody from Novus Biologicals
Targeting: NR1D1
ear-1, hRev, Rev-ErbAalpha, REVERBA, REVERBalpha, THRA1, THRAL
Western blot ELISA Immunocytochemistry Immunohistochemistry
Blocking/Neutralizing Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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- Product number
- H00009572-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00009572-M02, RRID:AB_715054
- Product name
- Mouse Monoclonal Rev-erb A alpha/NR1D1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. NR1D1 (4F6)
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references High expression of NR1D1 is associated with good prognosis in triple-negative breast cancer patients treated with chemotherapy.
Comparative cell cycle transcriptomics reveals synchronization of developmental transcription factor networks in cancer cells.
Ubiquitin ligase Siah2 regulates RevErbα degradation and the mammalian circadian clock.
Clock-controlled output gene Dbp is a regulator of Arnt/Hif-1β gene expression in pancreatic islet β-cells.
Na H, Han J, Ka NL, Lee MH, Choi YL, Shin YK, Lee MO
Breast cancer research : BCR 2019 Nov 28;21(1):127
Breast cancer research : BCR 2019 Nov 28;21(1):127
Comparative cell cycle transcriptomics reveals synchronization of developmental transcription factor networks in cancer cells.
Boström J, Sramkova Z, Salašová A, Johard H, Mahdessian D, Fedr R, Marks C, Medalová J, Souček K, Lundberg E, Linnarsson S, Bryja V, Sekyrova P, Altun M, Andäng M
PloS one 2017;12(12):e0188772
PloS one 2017;12(12):e0188772
Ubiquitin ligase Siah2 regulates RevErbα degradation and the mammalian circadian clock.
DeBruyne JP, Baggs JE, Sato TK, Hogenesch JB
Proceedings of the National Academy of Sciences of the United States of America 2015 Oct 6;112(40):12420-5
Proceedings of the National Academy of Sciences of the United States of America 2015 Oct 6;112(40):12420-5
Clock-controlled output gene Dbp is a regulator of Arnt/Hif-1β gene expression in pancreatic islet β-cells.
Nakabayashi H, Ohta Y, Yamamoto M, Susuki Y, Taguchi A, Tanabe K, Kondo M, Hatanaka M, Nagao Y, Tanizawa Y
Biochemical and biophysical research communications 2013 May 3;434(2):370-5
Biochemical and biophysical research communications 2013 May 3;434(2):370-5
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Analysis of NR1D1 over-expressed 293 cell line, cotransfected with NR1D1 Validated Chimera RNAi ( Cat # H00009572-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NR1D1 monoclonal antibody (M02), clone 4F6 (Cat # H00009572-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Analysis of NR1D1 expression in transfected 293T cell line by NR1D1 monoclonal antibody (M02), clone 4F6. Lane 1: NR1D1 transfected lysate (66.8 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - NR1D1 monoclonal antibody (M02), clone 4F6. Analysis of NR1D1 expression in NIH/3T3.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Detection limit for recombinant GST tagged NR1D1 is approximately 1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Analysis of monoclonal antibody to NR1D1 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - analysis of Rev-erb A alpha in paraffin embedded mouse lung sections using anti-Rev-erb A alpha antibody. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - analysis of NR1D1 in paraffin embedded mouse lung tissue using anti-NR1D1 antibody. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - analysis of NR1D1 in paraffin embedded human lung cancer tissue using anti-NR1D1 antibody. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Analysis of monoclonal antibody to NR1D1 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Staining on human breast cancer tissue. Image from verified customer review.
