Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [5]
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- Product number
- PA5-55601 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PPP1R8 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: ISTLTIEEGN LDIQRPKRKR KNSRVTFSED DEIINPEDVD PSVGRFRNMV QTAVVPVKKK RVEGPGSLGL EESGSRRMQN FAFSGGLY Highest antigen sequence identity to the following orthologs: Mouse - 98%, Rat - 97%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PPP1R8 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a PPP1R8 Polyclonal Antibody (Product # PA5-55601).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PPP1R8 in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a PPP1R8 Polyclonal Antibody (Product # PA5-55601). Remaining relative intensity is presented.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PPP1R8 using PPP1R8 Polyclonal Antibody (Product # PA5-55601) (A) shows similar pattern to an independent PPP1R8 Polyclonal Antibody (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of PPP1R8 in human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a PPP1R8 Polyclonal Antibody (Product # PA5-55601).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of PPP1R8 in human cell line A-431 using a PPP1R8 Polyclonal Antibody (Product # PA5-55601) shows localization to nuclear speckles.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PPP1R8 in human colon using PPP1R8 Polyclonal Antibody (Product # PA5-55601).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PPP1R8 in human lymph node using PPP1R8 Polyclonal Antibody (Product # PA5-55601).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PPP1R8 in human testis using PPP1R8 Polyclonal Antibody (Product # PA5-55601).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PPP1R8 in human cerebral cortex using PPP1R8 Polyclonal Antibody (Product # PA5-55601).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PPP1R8 in human thyroid gland using a PPP1R8 Polyclonal Antibody (Product # PA5-55601) shows strong nuclear positivity in glandular cells.