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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- 650803 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#650803, RRID:AB_10917386
- Product name
- Alexa Fluor® 488 anti-Histone H3 Phospho (Ser10) [11D8]
- Antibody type
- Monoclonal
- Antigen
- Modified synthetic peptide conjugated to KLH
- Description
- Histone H3 is phosphorylated at serine 10 during mitosis and found to be involved in transcriptional activation, chromatin decondensation, and chromosome compaction during cell division, by the action of Aurora kinase and NIMA kinases.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Vial size
- 25 tests
- Storage
- The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Hela cells were treated with 20 µM of Nocodazole for 24 hours then fixed and permeabilized with 70% ethanol and then stained with anti-Histone H3 Phospho-Ser10 (clone 11D8) Alexa Flour® 488, or mouse IgG2b, κ Alexa Flour® 488 isotype control.
- Conjugate
- Green dye
