Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF3168 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal HAND1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human HAND1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human HAND2 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Dissecting the dynamics of signaling events in the BMP, WNT, and NODAL cascade during self-organized fate patterning in human gastruloids.
Chhabra S, Liu L, Goh R, Kong X, Warmflash A
PLoS biology 2019 Oct;17(10):e3000498
PLoS biology 2019 Oct;17(10):e3000498
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human HAND1 by Western Blot. Western blot shows lysates of human heart tissue, human brain (cerebellum) tissue, and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HAND1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3168) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for HAND1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.