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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA502671 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502671, RRID:AB_11125431
- Product name
- HAND1 mouse monoclonal antibody, clone OTI1G10 (formerly 1G10)
- Antibody type
- Monoclonal
- Description
- HAND1 mouse monoclonal antibody, clone OTI1G10 (formerly 1G10)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- HAND1
- Isotype
- IgG
- Antibody clone number
- OTI1G10
- Vial size
- 100 µl
- Concentration
- 0.82 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HAND1 (RC200513, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HAND1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-HAND1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-HAND1 mouse monoclonal antibody (TA502671) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HAND1(RC200513).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-HAND1 antibody(TA502671),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
