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- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- ABIN954955 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Slingshot Homolog 3 (Drosophila) (SSH3) (C-Term), (AA 582-610) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 582-610 amino acids from the C-terminal region of Human SSH3 Genename: SSH3
- Description
- Protein A column, followed by peptide affinity purification
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- C-Term,AA 582-610
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Slingshot-3 dephosphorylates ADF/cofilin but is dispensable for mouse development.
The slingshot family of phosphatases mediates Rac1 regulation of cofilin phosphorylation, laminin-332 organization, and motility behavior of keratinocytes.
A probability-based approach for high-throughput protein phosphorylation analysis and site localization.
Interplay between components of a novel LIM kinase-slingshot phosphatase complex regulates cofilin.
Kousaka K, Kiyonari H, Oshima N, Nagafuchi A, Shima Y, Chisaka O, Uemura T
Genesis (New York, N.Y. : 2000) 2008 May;46(5):246-55
Genesis (New York, N.Y. : 2000) 2008 May;46(5):246-55
The slingshot family of phosphatases mediates Rac1 regulation of cofilin phosphorylation, laminin-332 organization, and motility behavior of keratinocytes.
Kligys K, Claiborne JN, DeBiase PJ, Hopkinson SB, Wu Y, Mizuno K, Jones JC
The Journal of biological chemistry 2007 Nov 2;282(44):32520-8
The Journal of biological chemistry 2007 Nov 2;282(44):32520-8
A probability-based approach for high-throughput protein phosphorylation analysis and site localization.
Beausoleil SA, Villén J, Gerber SA, Rush J, Gygi SP
Nature biotechnology 2006 Oct;24(10):1285-92
Nature biotechnology 2006 Oct;24(10):1285-92
Interplay between components of a novel LIM kinase-slingshot phosphatase complex regulates cofilin.
Soosairajah J, Maiti S, Wiggan O, Sarmiere P, Moussi N, Sarcevic B, Sampath R, Bamburg JR, Bernard O
The EMBO journal 2005 Feb 9;24(3):473-86
The EMBO journal 2005 Feb 9;24(3):473-86
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of SSH3 Antibody (C-term) Cat.-No AP54050PU-N in Hela cell line lysates (35ug/lane).This demonstrates the SSH3 antibody detected the SSH3 protein (arrow).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of SSH3 Antibody (C-term) Cat.-No AP54050PU-N with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Formalin fixed, paraffin embedded human breast carcinoma stained with SSH3 Antibody (C-term) Cat.-No AP54050PU-N, wihc was peroxidase conjugated to the secondary antibody and followed by DAB staining.This data demonstrates the use of SSH3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Hela cells using SSH3 Antibody (C-term) Cat.-No AP54050PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
