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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP51507PU-N - Provider product page
- Provider
- OriGene
- Product name
- FADS2 (N-term) rabbit polyclonal antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- FADS2 (N-term) rabbit polyclonal antibody, Aff - Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- FADS2
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- lot specific
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis in HepG2 cell line lysates (35ug/lane) using FADS2 Antibody (N-term) . This demonstrates the FADS2 antibody detected the FADS2 protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Fluorescent image of A549 cell stained with FADS2 Antibody (N-term) (Lot SA110322AN). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FADS2 primary antibody (1:25, 1 h at 37??). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37??).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37??). FADS2 immunoreactivity is localized to Cytoplasm and Vesicles significantly.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in Formalin Fixed, Paraffin Embedded Human liver tissue stained with FADS2 Antibody (N-term) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of FADS2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric analysis of K562 cells using FADS2 Antibody (N-term) (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis
- Validation comment
- FC
