Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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Validation data
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- Product number
- PA5-54947 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- WIPF2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: PVNIRTGPSG QSLAPPPPPY RQPPGVPNGP SSPTNESAPE LPQRHNSLHR KTPGPVRGLA PPPPTSASPS LLSNRPPP
- Concentration
- 0.2 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of WIPF2 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a WIPF2 Polyclonal Antibody (Product # PA5-54947).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of WIPF2 using WIPF2 Polyclonal Antibody (Product # PA5-54947) (A) shows similar pattern to an independent WIPF2 Polyclonal Antibody (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of WIPF2 in human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a WIPF2 Polyclonal Antibody (Product # PA5-54947).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of WIPF2 in human cerebral cortex using a WIPF2 Polyclonal Antibody (Product # PA5-54947) shows moderate positivity in neuropil.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of WIPF2 in human colon using WIPF2 Polyclonal Antibody (Product # PA5-54947) shows moderate cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of WIPF2 in human fallopian tube using WIPF2 Polyclonal Antibody (Product # PA5-54947) shows moderate cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of WIPF2 in human cerebral cortex using WIPF2 Polyclonal Antibody (Product # PA5-54947) shows moderate cytoplasmic positivity in neurons.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of WIPF2 in human liver using WIPF2 Polyclonal Antibody (Product # PA5-54947) shows no positivity in hepatocytes as expected.