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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Proximity ligation assay [3]
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- Product number
- H00000990-D01P - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00000990-D01P, RRID:AB_1673203
- Product name
- CDC6 purified MaxPab rabbit polyclonal antibody (D01P)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against a full-length human CDC6 protein.
- Antigen sequence
MPQTRSQAQATISFPKRKLSRALNKAKNSSDAKLE
PTNVQTVTCSPRVKALPLSPRKRLGDDNLCNTPHL
PPCSPPKQGKKENGPPHSHTLKGRRLVFDNQLTIK
SPSKRELAKVHQNKILSSVRKSQEITTNSEQRCPL
KKESACVRLFKQEGTCYQQAKLVLNTAVPDRLPAR
EREMDVIRNFLREHICGKKAGSLYLSGAPGTGKTA
CLSRILQDLKKELKGFKTIMLNCMSLRTAQAVFPA
IAQEICQEEVSRPAGKDMMRKLEKHMTAEKGPMIV
LVLDEMDQLDSKGQDVLYTLFEWPWLSNSHLVLIG
IANTLDLTDRILPRLQAREKCKPQLLNFPPYTRNQ
IVTILQDRLNQVSRDQVLDNAAVQFCARKVSAVSG
DVRKALDVCRRAIEIVESDVKSQTILKPLSECKSP
SEPLIPKRVGLIHISQVISEIDGNRMTLSQEGAQD
SFPLQQKILVCSLMLLIRQLKIKEVTLGKLYEAYS
KVCRKQQVAAVDQSECLSLSGLLEARGILGLKRNK
ETRLTKVFFKIEEKEIEHALKDKALIGNILATGLP- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of CDC6 expression in transfected 293T cell line (H00000990-T02) by CDC6 MaxPab polyclonal antibody.Lane 1: CDC6 transfected lysate(62.70 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of purified MaxPab antibody to CDC6 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Proximity Ligation Analysis of protein-protein interactions between CDC6 and MCM7. Huh7 cells were stained with anti-CDC6 rabbit purified polyclonal 1:600 and anti-MCM7 mouse monoclonal antibody 1:100. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Validation comment
- In situ Proximity Ligation Assay (Cell)
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Proximity Ligation Analysis of protein-protein interactions between CDC6 and CDKN1A. HeLa cells were stained with anti-CDC6 rabbit purified polyclonal 1:1200 and anti-CDKN1A mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Validation comment
- In situ Proximity Ligation Assay (Cell)
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Proximity Ligation Analysis of protein-protein interactions between CDC6 and PCNA. Mahlavu cells were stained with anti-CDC6 rabbit purified polyclonal 1:1200 and anti-PCNA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Validation comment
- In situ Proximity Ligation Assay (Cell)
