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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Other assay [2]
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- Product number
- TA347271 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal RARA Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal RARA Antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- RARA
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- not determined
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB using the antibody against RARA, diluted 1:750 in BSA/PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against human RARA. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:2,400.
- Validation comment
- ELISA
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ChIP assays were performed using NB4 cells, the antibody against RARA and optimized primer pairs for qPCR. Sheared chromatin from 6 million cells and 4 ul of antibody were used per ChIP experiment. QPCR was performed using primers specific for the TGM2, HMHA1, PRAM1 and H2B genes. Image shows the relative occupancy, calculated as the ratio + control/background for which the second exon of the MB gene was used.
- Validation comment
- Assay
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- a) ChIP-seq results obtained with the antibody against RARA ChIP was performed as described above and the IP'd DNA was analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Image shows the results of the complete chromosome 19 and two 50 kb region surrounding the HMHA1 and PRAM1 genes, respectively.
- Validation comment
- Assay
