F54372

antibody from NSJ Bioreagents

Targeting: RARA NR1B1, RAR

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54372

Provider

NSJ Bioreagents

Product name

RAR alpha Antibody / RARA

Antibody type

Polyclonal

Description

This highly specific RAR alpha antibody is suitable for use in Western blot/Flow cytometry/Immunohistochemistry/Immunofluorescence applications with human and mouse samples.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the RAR alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human HL60 and 2) mouse testis lysate with RAR alpha antibody. Predicted molecular weight ~51 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) HL60, 2) MCF7, 3) HepG2, 4) HeLa and 5) T-47D cell lysate with RAR alpha antibody. Predicted molecular weight ~51 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of fixed and permeabilized human MCF7 cells with Cytokeratin 18 antibody (green) and DAPI nuclear stain (blue).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE mouse cerebellum tissue with RAR alpha antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human HeLa cells with RAR alpha antibody; Blue=isotype control, Green= RAR alpha antibody.