ADR-003-200UL

antibody from Invitrogen Antibodies

Targeting: DRD3

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

ADR-003-200UL

Provider

Invitrogen Antibodies

Product name

D3 Dopamine Receptor (extracellular) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

200 µL

Concentration

0.8 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of whole rat brain (lanes 1 and 3) and striatum (lanes 2 and 4) membranes: - 1,2. Anti-D3Dopamine Receptor (extracellular) Antibody (#ADR-003), (1:200).3,4. Anti-D3Dopamine Receptor (extracellular) Antibody , preincubated with D3 Dopamine Receptor (extracellular) Blocking Peptide (#BLP-DR003).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of whole rat brain (lanes 1 and 3) and striatum (lanes 2 and 4) membranes: - 1,2. Anti-D3Dopamine Receptor (extracellular) Antibody (#ADR-003), (1:200).3,4. Anti-D3Dopamine Receptor (extracellular) Antibody , preincubated with D3 Dopamine Receptor (extracellular) Blocking Peptide (#BLP-DR003).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of mouse brain membranes: - 1. Anti-D3Dopamine Receptor (extracellular) Antibody (#ADR-003), (1:200). 2. Anti-D3Dopamine Receptor (extracellular) Antibody , preincubated with D3 Dopamine Receptor (extracellular) Blocking Peptide (#BLP-DR003).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of DRD3in rat striatum - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-D3Dopamine Receptor (extracellular) Antibody (#ADR-003), (1:100). A. DRD3appears in a subset of striatal neurons (green). B. The same section was stained for parvalbumin (red), a marker of interneurons. C. Merging the two images demonstrates that DRD3localization includes parvalbuminergic striatal interneurons. DAPI was used as the counterstain (blue).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Cell surface detection of D3 Dopamine Receptor by indirect flow cytometry in live intact human Jurkat T-cell leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-D3Dopamine Receptor (extracellular) Antibody (#ADR-003), (5μg) + goat- Anti-rabbit-FITC.