MAB8377

antibody from R&D Systems

Targeting: HHEX HEX, HOX11L-PEN, PRHX

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB8377

Provider

R&D Systems

Product name

Human HHEX Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human HHEX in direct ELISAs.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

Q03014


Isotype

IgG

Antibody clone number

906705

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human HHEX by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human HHEX Monoclonal Antibody (Catalog # MAB8377) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HHEX at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human HHEX by Simple Western^TM. Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.5 mg/mL. A specific band was detected for HHEX at approximately 47 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human HHEX Monoclonal Antibody (Catalog # MAB8377). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.