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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
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Validation data
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- Product number
- TA500026 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500026, RRID:AB_2056188
- Product name
- Anti-Hex (Hex) mouse monoclonal antibody, clone OTI3C4 (formerly 3C4)
- Antibody type
- Monoclonal
- Description
- Anti-Hex (Hex) mouse monoclonal antibody, clone OTI3C4 (formerly 3C4)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- HHEX
- Isotype
- IgG
- Antibody clone number
- OTI3C4
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Hex antibody (3C4) at 1:100 dilution + Lysates from HEK-293T cells transfected with human Hex expression vector (Cat# RC204815)
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HHex(RC204815) (RC204815, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HHex.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HHex monoclonal antibody at 1:50 dilution (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-HHex mouse monoclonal antibody (TA500026) immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY HHex(RC204815)
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-HHex mouse monoclonal antibody (TA500026).
- Validation comment
- IF
