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- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- R32788 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- ANGPTL4 Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific ANGPTL4 antibody is suitable for use in Western blot/Immunohistochemistry/ELISA applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the ANGPTL4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of recombinant human ANGPTL4 protein (1ng/lane) with ANGPTL4 antibody at 0.5ug/ml. Expected molecular weight: 50-55 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) placenta and 2) HCCT cell lysate with ANGPTL4 antibody. Predicted molecular weight: ~45 kDa and ~41 kDa, but may be observed at higher molecular weights due to glycosylation.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human spleen tissue with ANGPTL4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human placental tissue with ANGPTL4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
