AF3485

antibody from R&D Systems

Targeting: ANGPTL4 ARP4, FIAF, HFARP, NL2, PGAR, pp1158

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Antibody Data

Product number

AF3485

Provider

R&D Systems

Product name

Human/Primate Angiopoietin-like Protein 4/ANGPTL4 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human ANGPTL4 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human (rh) ANGPTL3, rhAngiopoietin-1, -2, -3, and -4 is observed. In Western blots, approximately 1% cross-reactivity with rhANGPLT4 N-terminal Fragment is observed.

Reactivity

Human, Simian

Host

Goat

Conjugate

Unconjugated

Antigen sequence

Q9BY76


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Regulation of angiopoietin-like 4 and lipoprotein lipase in human adipose tissue.

Dijk W, Schutte S, Aarts EO, Janssen IMC, Afman L, Kersten S
Journal of clinical lipidology 2018 May - Jun;12(3):773-783

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Buyang Huanwu Tang improves denervation-dependent muscle atrophy by increasing ANGPTL4, and increases NF-κB and MURF1 levels.

Zhou L, Huang Y, Xie H, Mei X
Molecular medicine reports 2018 Mar;17(3):3674-3680

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Plasma angiopoietin-like 4 is related to phospholipid transfer protein activity in diabetic and non-diabetic subjects: role of enhanced low grade inflammation.

Gruppen EG, Kersten S, Dullaart RPF
Lipids in health and disease 2018 Mar 27;17(1):60

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Effect of diet-induced weight loss on angiopoietin-like protein 4 and adipose tissue lipid metabolism in overweight and obese humans.

van der Kolk BW, Vink RG, Jocken JWE, Roumans NJT, Goossens GH, Mariman ECM, van Baak MA, Blaak EE
Physiological reports 2018 Jul;6(13):e13735

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ANGPTL4 promotes the progression of cutaneous melanoma to brain metastasis.

Izraely S, Ben-Menachem S, Sagi-Assif O, Meshel T, Marzese DM, Ohe S, Zubrilov I, Pasmanik-Chor M, Hoon DSB, Witz IP
Oncotarget 2017 Sep 29;8(44):75778-75796

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Hypoxia-induced ANGPTL4 sustains tumour growth and anoikis resistance through different mechanisms in scirrhous gastric cancer cell lines.

Baba K, Kitajima Y, Miyake S, Nakamura J, Wakiyama K, Sato H, Okuyama K, Kitagawa H, Tanaka T, Hiraki M, Yanagihara K, Noshiro H
Scientific reports 2017 Sep 11;7(1):11127

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Short-chain fatty acids stimulate angiopoietin-like 4 synthesis in human colon adenocarcinoma cells by activating peroxisome proliferator-activated receptor γ.

Alex S, Lange K, Amolo T, Grinstead JS, Haakonsson AK, Szalowska E, Koppen A, Mudde K, Haenen D, Al-Lahham S, Roelofsen H, Houtman R, van der Burg B, Mandrup S, Bonvin AM, Kalkhoven E, Müller M, Hooiveld GJ, Kersten S
Molecular and cellular biology 2013 Apr;33(7):1303-16

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Caloric restriction and exercise increase plasma ANGPTL4 levels in humans via elevated free fatty acids.

Kersten S, Lichtenstein L, Steenbergen E, Mudde K, Hendriks HF, Hesselink MK, Schrauwen P, Müller M
Arteriosclerosis, thrombosis, and vascular biology 2009 Jun;29(6):969-74