Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF804 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IGFBP-4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human IGFBP-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse IGFBP-4 is observed, and less than 1% cross-reactivity with recombinant human (rh) IGFBP-1, rhIGFBP-2, rhIGFBP-3, rhIGFBP-5 and rhIGFBP-6 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
AAA62670
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references EWS-FLI-1 creates a cell surface microenvironment conducive to IGF signaling by inducing pappalysin-1.
Fetal baboon sex-specific outcomes in adipocyte differentiation at 0.9 gestation in response to moderate maternal nutrient reduction.
IGFBP-4 and -5 are expressed in first-trimester villi and differentially regulate the migration of HTR-8/SVneo cells.
The role of matrix metalloproteinase-7 in redefining the gastric microenvironment in response to Helicobacter pylori.
Jayabal P, Houghton PJ, Shiio Y
Genes & cancer 2017 Nov;8(11-12):762-770
Genes & cancer 2017 Nov;8(11-12):762-770
Fetal baboon sex-specific outcomes in adipocyte differentiation at 0.9 gestation in response to moderate maternal nutrient reduction.
Tchoukalova YD, Krishnapuram R, White UA, Burk D, Fang X, Nijland MJ, Nathanielsz PW
International journal of obesity (2005) 2014 Feb;38(2):224-30
International journal of obesity (2005) 2014 Feb;38(2):224-30
IGFBP-4 and -5 are expressed in first-trimester villi and differentially regulate the migration of HTR-8/SVneo cells.
Crosley EJ, Dunk CE, Beristain AG, Christians JK
Reproductive biology and endocrinology : RB&E 2014 Dec 4;12:123
Reproductive biology and endocrinology : RB&E 2014 Dec 4;12:123
The role of matrix metalloproteinase-7 in redefining the gastric microenvironment in response to Helicobacter pylori.
McCaig C, Duval C, Hemers E, Steele I, Pritchard DM, Przemeck S, Dimaline R, Ahmed S, Bodger K, Kerrigan DD, Wang TC, Dockray GJ, Varro A
Gastroenterology 2006 May;130(6):1754-63
Gastroenterology 2006 May;130(6):1754-63
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IGFBP-4 in Human Placenta. IGFBP-4 was detected in immersion fixed paraffin-embedded sections of human placenta (chorionic villi) using 5 µg/mL Goat Anti-Human IGFBP-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF804) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IGFBP-4 Inhibition of IGF-II-dependent Cell Proliferation and Neutralization by Human IGFBP-4 Antibody. Recombinant Human IGFBP-4 (Catalog # 804-GB) inhibits Recombinant Human IGF-II (Catalog # 292-G2) induced proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IGF-II (14 ng/mL) activity elicited by Recombinant Human IGFBP-4 (0.3 μg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IGFBP-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF804). The ND50 is typically 12-60 µg/mL.