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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00084951-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00084951-M01, RRID:AB_426105
- Product name
- TNS4 monoclonal antibody (M01), clone 3B8
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant TNS4.
- Antigen sequence
MGSKASSPHGLGSPLVASPRLEKRLGGLAPQRGSR
ISVLSASPVSDVSYMFGSSQSLLHSSNSSHQSSSR
SLESPANSSSSLHSLGSVSLCTRPSDFQAPRNPTL
TMGQPRTPHSPPLAKEHASSCPPSITNSMVDIPIV
LINGCPEPGSSPPQRTPGHQNSVQPGAASPSNPCP
ATRSNSQTLSDAPFTTCPEGPARDMQPTMKFVMDT
SKYWFKPNITREQAIELLRKEEPGAFVIRDSSSYR
GSFGLALKVQEVPASAQSRPGEDSNDLIRHFLIES
SAKGVHLKGADEEPYFGSLSAFVCQHSIMALALPC
KLTIPQRELGGADGASDSTDSPASCQKKSAGCHTL
YLSSVSVETLTGALAVQKAISTTFERDILPTPTVV
HFKVTEQGITLTDVQRKVFFRRHYPLTTLRFCGMD
PEQRKWQKYCKPSWIFGFVAKSQTEPQENVCHLFA
EYDMVQPASQVIGLVTALLQDAERM- Isotype
- IgG
- Antibody clone number
- 3B8
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Loss of the integrin-activating transmembrane protein Fam38A (Piezo1) promotes a switch to a reduced integrin-dependent mode of cell migration.
Prognostic relevance of Tensin4 expression in human gastric cancer.
McHugh BJ, Murdoch A, Haslett C, Sethi T
PloS one 2012;7(7):e40346
PloS one 2012;7(7):e40346
Prognostic relevance of Tensin4 expression in human gastric cancer.
Sakashita K, Mimori K, Tanaka F, Kamohara Y, Inoue H, Sawada T, Hirakawa K, Mori M
Annals of surgical oncology 2008 Sep;15(9):2606-13
Annals of surgical oncology 2008 Sep;15(9):2606-13
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of TNS4 expression in transfected 293T cell line by TNS4 monoclonal antibody (M01), clone 3B8.Lane 1: TNS4 transfected lysate(51.8 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged TNS4 is approximately 0.1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to TNS4 on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
