Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- MAB6925 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Tensin 4/CTEN Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Tensin 4/CTEN in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q8IZW8
- Isotype
- IgG
- Antibody clone number
- 684524
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references C-terminal tensin-like protein is a novel prognostic marker for primary melanoma patients.
Sjoestroem C, Khosravi S, Zhang G, Martinka M, Li G
PloS one 2013;8(11):e80492
PloS one 2013;8(11):e80492
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Tensin 4/CTEN by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Tensin 4/CTEN at approximately 74 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Tensin 4/CTEN Monoclonal Antibody (Catalog # MAB6925). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Tensin 4/CTEN in Human Prostate. Tensin 4/CTEN was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human Tensin 4/ CTEN Monoclonal Antibody (Catalog # MAB6925) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Tensin 4/CTEN by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Tensin 4/CTEN Monoclonal Antibody (Catalog # MAB6925) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Tensin 4/CTEN at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.