GTX629629

antibody from GeneTex

Targeting: FASN FAS, SDR27X1

Immunocytochemistry

Antibody data

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Validation data

FASN protein structure - GTX629629 shown in red.

Enhanced validation

Submitted by: GeneTex (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [GT325] (GTX629629) diluted at 1:500.
KD Reagent Type: siRNA
Downregulation: >50%
KD Reagent Prod page: KD Reagent Prod page
KD Reagent Provider: shRNA reagents was provided courtesy of cooperative labs.
KD Reagent Prod no: Proprietary.
Antibody Lot Number: 41414

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Fatty Acid Synthase antibody detects Fatty Acid Synthase protein by Western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g A431 whole cell lysate/extractC. 30 ?g HepG2 whole cell lysate/extract5 % SDS-PAGEFatty Acid Synthase antibody (GTX629629) dilution: 1:1000

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Fatty Acid Synthase antibody detects Fatty Acid Synthase protein by Western blot analysis.A. 30 ?g Neuro2A whole cell lysate/extract B. 30 ?g GL261 whole cell lysate/extractC. 30 ?g BCL-1 whole cell lysate/extract5 % SDS-PAGEFatty Acid Synthase antibody (GTX629629) dilution: 1:500

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Fatty Acid Synthase antibody detects Fatty Acid Synthase protein by Western blot analysis.A. 30 ?g PC-12 whole cell lysate/extractB. 30 ?g Rat-2 whole cell lysate/extract5 % SDS-PAGEFatty Acid Synthase antibody (GTX629629) dilution: 1:5000

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [GT325] (GTX629629) diluted at 1:500.