GTX629762

antibody from GeneTex

Targeting: FASN FAS, SDR27X1

Western blot (Enhanced validation)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX629762

Provider

GeneTex

Product name

Fatty Acid Synthase antibody [GT556]

Antibody type

Monoclonal

Reactivity

Human, Mouse, Rat

Host

Mouse

Western blot

Enhanced validation

Submitted by

GeneTex (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [GT556] (GTX629762) diluted at 1:500.

KD Reagent Type

siRNA

Downregulation

>75%

KD Reagent Prod page

KD Reagent Prod page

KD Reagent Provider

shRNA reagents was provided courtesy of cooperative labs.

KD Reagent Prod no

Proprietary.

Antibody Lot Number

41435

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Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Fatty Acid Synthase antibody [GT556] detects Fatty Acid Synthase protein by Western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g A431 whole cell lysate/extract C. 30 ?g HeLa whole cell lysate/extractD. 30 ?g HepG2 whole cell lysate/extract5 % SDS-PAGEFatty Acid Synthase antibody [GT556] (GTX629762) dilution: 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [GT556] (GTX629762) diluted at 1:500.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Fatty Acid Synthase antibody [GT556] detects Fatty Acid Synthase protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Fatty Acid Synthase antibody [GT556] (GTX629762) diluted at 1:250.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Fatty Acid Synthase antibody [GT556] detects Fatty Acid Synthase protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. Fatty Acid Synthase antibody [GT556] (GTX629762) diluted at 1:250.