PA1-32355

antibody from Invitrogen Antibodies

Targeting: FASN FAS, SDR27X1

Immunoprecipitation

Antibody data

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Validation data

Product number: PA1-32355 - Provider product page
Provider: Invitrogen Antibodies
Product name: FASN Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: PA1-32355 detects Fatty Acid Synthase from mouse, porcine, human, monkey and rat samples.
Reactivity: Human, Mouse, Rat, Chicken/Avian, Hamster, Porcine
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: Conc. Not Determined
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

FASN protein structure - PA1-32355 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot detection of Fatty Acid Synthase in chicken hypothalamus. A: total RNA (1 µg) isolated from different tissues (brain, hypothalamus, liver and muscle) was subjected to RT-PCR using specific primers for chicken Fatty Acid Synthase or ribosomal 18S as a control. B: tissue lysates (brain, hypothalmus, liver and muscle) probed using a Fatty Acid Synthase polyclonal antibody (Product # PA1-32355) and detected using enhanced chemiluminescence.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Fatty Acid Synthase in 50mg of total mouse liver lysate. Samples were resolved on a 4-15% SDS polyacrylamide gel and probed with a Fatty Acid Synthase polyclonal antibody (Product # PA1-32355) followed by ECL detection (1 second exposure).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of FASN was performed by loading 50 µg of mouse liver tissue lysate. Proteins were transferred to a membrane and probed with a FASN Polyclonal Antibody (Product # PA1-32355).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of FASN was achieved by transfecting Hep G2 with FASN specific siRNAs (Silencer® select Product # S5031, S5032). Western blot analysis (Fig. a) was performed using Whole cell extracts from the FASN knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with FASN Polyclonal Antibody (Product # PA1-32355, 1:1000 ) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to FASN.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-FASN Polyclonal Antibody (Product # PA1-32355) and a ~260kDa band corresponding to FASN was observed across cell lines tested . Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), A549 (Lane 2), HeLa (Lane 3), Caco-2 (Lane 4), NTERA-2 cl.D1 (Lane 5), PC-3 (Lane 6) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).