AM2067b

antibody from Abgent

Targeting: FASN FAS, SDR27X1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM2067b

Provider

Abgent

Product name

FASN Antibody (Center)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Mouse Monoclonal Antibody (Mab)

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgM

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

FASN Antibody (Center) (Cat. #AM2067b) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the FASN (Center) antibody detected the FASN (Center) protein (arrow).

Primary Ab dilution

1:100

Immunocytochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Fluorescent confocal image of HepG2 cells stained with FASN (Center) antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AM2067b FASN primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ¼g/ml, 5 min). Note the highly specific localization of the FASN immunosignal to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000169710)."

Primary Ab dilution

1:200