Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB59271 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Fatty Acid Synthase/FASN Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects Fatty Acid Synthase/FASN in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse (rm) Ephrin-A2 is observed and no cross-reactivity with recombinant human (rh) Ephrin-A3, -B3, rmEphrin-A4, -A5, -B1 or -B2 is observed.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Human Fatty Acid Synthase/FASN by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and HEK293 human embryonic kidney cell line. PVDF Membrane was probed with 0.25 µg/mL of Mouse Anti-Human Fatty Acid Synthase/FASN Monoclonal Antibody (Catalog # MAB59271) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Fatty Acid Synthase/FASN at approximately 220 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.