TA324352

antibody from OriGene

Targeting: FASN FAS, SDR27X1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA324352

Provider

OriGene

Product name

Mouse monoclonal FASN Antibody (Center)

Antibody type

Monoclonal

Description

Mouse monoclonal FASN Antibody (Center)

Host

Mouse

Conjugate

Unconjugated

Epitope

FASN

Isotype

IgM

Antibody clone number

497CT15.2.5

Vial size

400 µl

Concentration

2.0 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

FASN Antibody (Center) (Cat. #TA324352) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the FASN (Center) antibody detected the FASN (Center) protein (arrow).

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

IF image of HepG2 cells stained with FASN (Center) antibody. HepG2 cells were incubated with TA324352 FASN primary antibody (1:200, 2 h at RT). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) . Note the highly specific localization of the FASN immunosignal to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000169710).

Validation comment

IF