Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- LS-C408077 - Provider product page
- Provider
- LSBio
- Product name
- FASN / Fatty Acid Synthase Antibody (aa1-226) LS-C408077
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified
- Reactivity
- Human
- Host
- Rabbit
- Storage
- At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis of FASN expression in HEPG2 whole cell lysates (lane 1), U87 whole cell lysates (lane 2) and RAJI whole cell lysates (lane 3). FASN at 273 kD was detected using rabbit anti- FASN Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis of FASN expression in HEPG2 whole cell lysates (lane 1), U87 whole cell lysates (lane 2) and RAJI whole cell lysates (lane 3). FASN at 273KD was detected using rabbit anti-FASN Antigen Affinity purified polyclonal antibody at0.5 ug/ml. The blot was developed using chemiluminescence (ECL) method.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- FASN was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti- FASN Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC analysis of FASN using anti-FASN antibody. FASN was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-FASN Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC analysis of FASN using anti-FASN antibody. FASN was detected in immunocytochemical section of MCF-7 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-FASN Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.