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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- ARG55884 - Provider product page
- Provider
- Arigo
- Product name
- anti-Fatty Acid Synthase antibody
- Antibody type
- Polyclonal
- Antigen
- KLH-conjugated synthetic peptide around aa. 942-973 (Center) of Human FASN.
- Description
- This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 35 µg of Ramos cell line lysate stained with ARG55884 anti-Fatty Acid Synthase antibody.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 2 µg of 293 cell lysates stained with ARG55884 anti-Fatty Acid Synthase antibody. The cells either nontransfected (Lane 1) or transiently transfected with the FASN gene (Lane 2). (Origene Technologies).
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 20 µg of 1) A549, 2) HeLa, 3) HepG2, and 4) SH-SY5Y whole cell lysates stained with ARG55884 anti-Fatty Acid Synthase antibody at 1:16000 dilution. Blocking/Dilution buffer: 5% NFDM/TBST.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: Confocal image of HepG2 cells stained with ARG55884 anti-Fatty Acid Synthase antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with primary antibody (1:200, 2 h at room temperature). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). Note the highly specific localization of the FASN mainly to the mainly to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000169710).
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: HeLa cell stained with ARG55884 anti-Fatty Acid Synthase antibody (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: formalin-fixed and paraffin-embedded Human placenta tissue stained with ARG55884 anti-Fatty Acid Synthase antibody.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Flow Cytometry: HeLa cells stained with ARG55884 anti-Fatty Acid Synthase antibody (right histogram) or without primary antibody control (left histogram), followed by incubation with FITC labelled secondary antibody.
