H00002194-M02J

antibody from Abnova Corporation

Targeting: FASN FAS, SDR27X1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00002194-M02J

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00002194-M02J, RRID:AB_1136880

Product name

FASN monoclonal antibody (M02J), clone 3B3-1D6

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant FASN.

Antigen sequence

MSTNDTIVSGTLPQRMASCLEVLDLFLNQPHMVLS
SFVLAEKAAAYRDRDSQRDLVEAVAHILGIRDLAA
VNLDSSLADLGLDSLMSVEVRQTLERELNLVLSVR
EVRQLTLRKLQELSSKADEASELACPTPKEDGLAQ
QQTQLNLRSLLVNPEGPTLMRLNSVQSSERPLFLV
HPIEGSTTVFHSLASGLSIPTYGLQCTRAAPLDSI
HSLAAYYIDCIRQVQPEGPYRVAGYSYGACVAFEM
CSQLQAQQSPAPTHNSLFLFDGSPTYVLAYTQSYR
AKLTPGCEAEAETEAICFFVQQFTDMEHNRVLEAL
LPLKGLEERVAAAVDLIIKSHQGLDRQELSFAARS
FYYKLRAAEQYTPKAKYHGNVMLLRAKTGGAYGED
LGADYNLSQVCDGKVSVHVIEGDHRTLLEGSGLES
IISIIHSSLAEPRVSVREG


Isotype

IgG

Antibody clone number

3B3-1D6

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of FASN expression in transfected 293T cell line by FASN monoclonal antibody (M02A), clone 3B3-1D6.Lane 1: FASN transfected lysate(48.3 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged FASN is 0.1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol