PA5-121085

antibody from Invitrogen Antibodies

Targeting: CEBPG GPE1BP, IG/EBP-1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-121085

Provider

Invitrogen Antibodies

Product name

C/EBP gamma Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive test controls include: A-549, HT-29, Mouse liver, Mouse testis, Mouse lung. The target is usually found in the following locations: Nucleus. Immunogen sequence: MSKISQQNST PGVNGISVIH TQAHASGLQQ VPQLVPAGPG GGGKAVAPSK QSKKSSPMDR NSDEYRQRRE RNNMAVKKSR LKSKQKAQDT LQRVNQLKEE NERLEAKIKL LTKELSVLKD LFLEHAHNLA DNVQSISTEN TTADGDNAGQ

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.48 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of C/EBP gamma in extracts of various cell lines. Samples were incubated with C/EBP gamma Polyclonal antibody (Product # PA5-121085) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of C/EBP gamma in A-549 cells. Samples were incubated with C/EBP gamma Polyclonal antibody (Product # PA5-121085).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of C/EBP gamma in paraffin-embedded human colon carcinoma. Samples were incubated with C/EBP gamma Polyclonal antibody (Product # PA5-121085) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of C/EBP gamma in paraffin-embedded human kidney. Samples were incubated with C/EBP gamma Polyclonal antibody (Product # PA5-121085) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of C/EBP gamma in paraffin-embedded human kidney cancer. Samples were incubated with C/EBP gamma Polyclonal antibody (Product # PA5-121085) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of C/EBP gamma in paraffin-embedded mouse liver. Samples were incubated with C/EBP gamma Polyclonal antibody (Product # PA5-121085) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of C/EBP gamma in paraffin-embedded human kidney cancer. Samples were incubated with C/EBP gamma Polyclonal antibody (Product # PA5-121085) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of C/EBP gamma in paraffin-embedded mouse liver. Samples were incubated with C/EBP gamma Polyclonal antibody (Product # PA5-121085) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.