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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- GTX113217 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX113217, RRID:AB_2037282
- Product name
- ITK antibody [N2C1], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: Molt-4 (GTX27912) 7.5% SDS PAGE GTX113217 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ITK antibody (GTX113217) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ITK antibody [N2C1], Internal detects ITK protein at cytoplasm by immunofluorescent analysis.Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ITK protein stained by ITK antibody [N2C1], Internal (GTX113217) diluted at 1:500.Blue: Hoechst 33342 staining.
