14-9015-80

antibody from Invitrogen Antibodies

Targeting: BTK AGMX1, ATK, IMD1, PSCTK1, XLA

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14-9015-80

Provider

Invitrogen Antibodies

Product name

Phospho-BTK/ITK (Tyr551, Tyr511) Monoclonal Antibody (M4G3LN), eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Immunogen sequence: MEGVLYKWT NYLTGWQPRW FVLDNGILSY YDSQDDVCKG SKGSIKMAVC EIKVHSADNT RMELIIPGEQ HFYMKAVNAA ERQRWLVALG SSKACLTDTR TKKEKEISET SESLKTKMSE LRLYCDLLMQ QVHTIQEFVH HDENHSSPSA ENMNEASSLL SATCNTFITT LEECVKIANA KFKPEMFQLH HPDPLVSPVS PSPVQMMKRS VSHPGSCSSE RSSHSIKEPV STLHRLSQRR RRTYSDTDSC SDIPLEDPDR PVHCSKNTLN GDLASATIPE ESRLMAKKQS ESEDTLPSFS S (1-300 aa encoded by BC044567)

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

M4G3LN

Vial size

25 µg

Concentration

0.5 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoblotting of reduced lysates from normal human peripheral blood cells treated with hydrogen peroxide-activated sodium orthovanadate (lane 1) or left untreated (lane 2), and probed with 5 µg/mL of Anti-Human/Mouse Phospho-BTK/ITK (Y551/Y511) Purified.Bands were visualized using Rat Anti-Mouse IgG HRP.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of formalin-fixed paraffin embedded human tonsil using 0.5 µg/mL of Anti-Human/Mouse Phospho-BTK/ITK (Y551/Y511) Purified, followed by Anti-Mouse IgG Biotin, Streptavidin HRP and DAB visualization.Nuclei are counterstained with hematoxylin.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 8 Enrichment and activation of B cell-associated signaling pathways in HS skin. Analysis of the signal transduction networks using literature-based networks (Genomatix-Pathway System, GePS) demonstrated enrichment for pathways involved in B cell signaling and activation ( A ). To confirm the nature of the inflammatory infiltrate in HS and the localization of components of the enriched signaling pathways, we performed IHC in an excisional biopsy for CD3, CD20, and CD138. Plasma cells were the predominant inflammatory infiltrate and most prominent in the deeper layers of the skin surrounding a deeper sinus tract ( A ), accompanied by increased expression of BTK, SYK, and LCK ( B ) ( n = 3). Activation of key components of this signaling pathway was confirmed by IHC for both phospho-BTK and phospho-SYK ( n = 3) ( C ).